Name: Anti-MYO7A Antibody (CAB1911)
Brand: Cell Biology Antibodies 7
SKU: CAB1911
Availability: OutOfStock

Antibody Name:	Anti-MYO7A Antibody
Antibody SKU:	CAB1911
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 850-1150 of human MYO7A (NP_000251.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 850-1150 of human MYO7A (NP_000251.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MIAR RLHQ RLRA EYLW RLEA EKMR LAEE EKLR KEMS AKKA KEEA ERKH QERL AQLA REDA EREL KEKE AARR KKEL LEQM ERAR HEPV NHSD MVDK MFGF LGTS GGLP GQEG QAPS GFED LERG RREM VEED LDAA LPLP DEDE EDLS EYKF AKFA ATYF QGTT THSY TRRP LKQP LLYH DDEG DQLA ALAV WITI LRFM GDLP EPKY HTAM SDGS EKIP VMTK IYET LGKK TYKR ELQA LQGE GEAQ LPEG QKKS SVRH KLVH LTLK KKSK LTEE VTKR LHDG ESTV QGNS MLED RPTS N
Gene ID:	4647
Uniprot:	Q13402
Cellular Location:	Cytoplasm, cell cortex, cytoskeleton
Calculated MW:	138kDa/240kDa/249kDa/250kDa/254kDa
Observed MW:	254kDa

Synonyms:

MYO7A, DFNA11, DFNB2, MYOVIIA, MYU7A, NSRD2, USH1B

Background:

This gene is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain, an actin-binding domain, a neck domain that interacts with other proteins, and a tail domain that serves as an anchor. This gene encodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness, reduced vestibular function, and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	MYO7A: an actin-based motor molecule with ATPase activity and a calcium sensitive calmodulin binding subunit. May play a role in trafficking of ribbon- synaptic vesicle complexes and renewal of outer photoreceptor disks. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity. Seven alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Motor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11q13.5 Cellular Component: stereocilium; photoreceptor inner segment; photoreceptor outer segment; lysosomal membrane; cytoplasm; apical plasma membrane; melanosome; synapse; cell cortex; cytosol; photoreceptor connecting cilium Molecular Function:actin filament binding; microfilament motor activity; calmodulin binding; protein domain specific binding; protein binding; protein homodimerization activity; spectrin binding; ADP binding; actin-dependent ATPase activity; ATP binding Biological Process: phagolysosome formation; intracellular protein transport; eye photoreceptor cell development; sensory perception of sound; pigment granule transport; visual perception; actin filament-based movement; lysosome organization and biogenesis; metabolic process; sensory perception of light stimulus; auditory receptor cell stereocilium organization and biogenesis; equilibrioception; post-embryonic organ morphogenesis Disease: Deafness, Autosomal Dominant 11; Deafness, Autosomal Recessive 2; Usher Syndrome, Type I
NCBI Summary:	This gene is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain, an actin-binding domain, a neck domain that interacts with other proteins, and a tail domain that serves as an anchor. This gene encodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness, reduced vestibular function, and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13402
NCBI GenInfo Identifier:	460018219
NCBI Gene ID:	4647
NCBI Accession:	Q13402.2
UniProt Related Accession:	Q13402
Molecular Weight:
NCBI Full Name:	Unconventional myosin-VIIa
NCBI Synonym Full Names:	myosin VIIA
NCBI Official Symbol:	MYO7A
NCBI Official Synonym Symbols:	DFNB2; MYU7A; NSRD2; USH1B; DFNA11; MYOVIIA
NCBI Protein Information:	unconventional myosin-VIIa
UniProt Protein Name:	Unconventional myosin-VIIa
Protein Family:	Unconventional myosin
UniProt Gene Name:	MYO7A
UniProt Entry Name:	MYO7A_HUMAN

Western blot analysis of extracts of various cell lines, using MYO7A antibody (CAB1911) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

MYO7A Antibody (PACO10703)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)