Name: Anti-MYO1E Antibody (CAB13032)
Brand: Cell Biology Antibodies 3
SKU: CAB13032
Availability: OutOfStock

Antibody Name:	Anti-MYO1E Antibody
Antibody SKU:	CAB13032
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 809-1108 of human MYO1E (NP_004989.2).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 809-1108 of human MYO1E (NP_004989.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KEVL KRKI EIER ILSV SLST MQDD IFIL HEQE YDSL LESV FKTE FLSL LAKR YEEK TQKQ LPLK FSNT LELK LKKE NWGP WSAG GSRQ VQFH QGFG DLAV LKPS NKVL QVSI GPGL PKNS RPTR RNTT QNTG YSSG TQNA NYPV RAAP PPPG YHQN GVIR NQYV PYPH APGS QRSN QKSL YTSM ARPP LPRQ QSTS SDRV SQTP ESLD FLKV PDQG AAGV RRQT TSRP PPAG GRPK PQPK PKPQ VPQC KALY AYDA QDTD ELSF NAND IIDI IKED PSGW WTGR LRGK QGLF PNNY VTKI
Gene ID:	4643
Uniprot:	Q12965
Cellular Location:	Cell junction, Cytoplasm, Cytoplasmic vesicle, clathrin-coated vesicle, cytoskeleton
Calculated MW:	127kDa
Observed MW:	127kDa

Synonyms:

MYO1E, FSGS6, HuncM-IC, MYO1C, myosin IE

Background:

This gene encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. Class I myosins are characterized by a head (motor) domain, a regulatory domain and a either a short or long tail domain. Among the class I myosins, this protein is distinguished by a long tail domain that is involved in crosslinking actin filaments. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in this gene are the cause of focal segmental glomerulosclerosis-6. This gene has been referred to as myosin IC in the literature but is distinct from the myosin IC gene located on chromosome 17.

UniProt Protein Function:	MYO1E: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. Binds to membranes containing anionic phospholipids via its tail domain. Required for normal morphology of the glomerular basement membrane, normal development of foot processes by kidney podocytes and normal kidney function. In dendritic cells, may control the movement of class II-containing cytoplasmic vesicles along the actin cytoskeleton by connecting them with the actin network via ARL14EP and ARL14. Defects in MYO1E are the cause of focal segmental glomerulosclerosis type 6 (FSGS6). A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. FSGS6 is a childhood-onset disorder resulting in nephrotic syndrome, which includes massive proteinuria, hypoalbuminemia, hyperlipidemia, and edema.
UniProt Protein Details:	Protein type:Actin-binding; Motor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 15q21-q22 Cellular Component: actin cytoskeleton; adherens junction; clathrin-coated endocytic vesicle; cytoplasm; cytoskeleton; intercellular junction; myosin complex Molecular Function:actin filament binding; ATPase activity, coupled; calmodulin binding; microfilament motor activity; motor activity; phosphoinositide binding; protein binding Biological Process: actin filament-based movement; endocytosis; glomerular basement membrane development; glomerular filtration Disease: Focal Segmental Glomerulosclerosis 6
NCBI Summary:	This gene encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. Class I myosins are characterized by a head (motor) domain, a regulatory domain and a either a short or long tail domain. Among the class I myosins, this protein is distinguished by a long tail domain that is involved in crosslinking actin filaments. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in this gene are the cause of focal segmental glomerulosclerosis-6. This gene has been referred to as myosin IC in the literature but is distinct from the myosin IC gene located on chromosome 17. [provided by RefSeq, Jan 2012]
UniProt Code:	Q12965
NCBI GenInfo Identifier:	215274106
NCBI Gene ID:	4643
NCBI Accession:	Q12965.2
UniProt Secondary Accession:	Q12965,Q14778,
UniProt Related Accession:	Q12965
Molecular Weight:	127,062 Da
NCBI Full Name:	Unconventional myosin-Ie
NCBI Synonym Full Names:	myosin IE
NCBI Official Symbol:	MYO1E
NCBI Official Synonym Symbols:	FSGS6; MYO1C; HuncM-IC
NCBI Protein Information:	unconventional myosin-Ie
UniProt Protein Name:	Unconventional myosin-Ie
UniProt Synonym Protein Names:	Myosin-Ic; Unconventional myosin 1E
Protein Family:	Unconventional myosin
UniProt Gene Name:	MYO1E
UniProt Entry Name:	MYO1E_HUMAN

Western blot analysis of extracts of HeLa cells, using MYO1E antibody (CAB13032) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

MYO1E Antibody (PACO10702)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)