Antibody Name:	Anti-MYO10 Antibody
Antibody SKU:	CAB12471
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 845-944 of human MYO10 (NP_036466.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	U-87MG, Mouse kidney, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 845-944 of human MYO10 (NP_036466.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EAEL RAQQ EEET RKQQ ELEA LQKS QKEA ELTR ELEK QKEN KQVE EILR LEKE IEDL QRMK EQQE LSLT EASL QKLQ ERRD QELR RLEE EACR AAQE FLES
Gene ID:	4651
Uniprot:	Q9HD67
Cellular Location:	Cell projection, Cytoplasm, Peripheral membrane protein, cell cortex, cytoskeleton, cytosol, filopodium membrane, filopodium tip, lamellipodium, ruffle
Calculated MW:	11kDa/163kDa/237kDa
Observed MW:	270kDa

Synonyms:

MYO10, myosin X

Background:

This gene encodes a member of the myosin superfamily. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-10 (MYH10). Unconventional myosins contain the basic domains of conventional myosins and are further distinguished from class members by their tail domains. This gene functions as an actin-based molecular motor and plays a role in integration of F-actin and microtubule cytoskeletons during meiosis.

UniProt Protein Function:	MYO10: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. MYO10 binds to actin filaments and actin bundles and functions as plus end-directed motor. The tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate or integrins, and mediates cargo transport along actin filaments. Regulates cell shape, cell spreading and cell adhesion. Stimulates the formation and elongation of filopodia. May play a role in neurite outgrowth and axon guidance. Plays a role in formation of the podosome belt in osteoclasts.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 5p15.1 Cellular Component: cytosol; filopodium tip; nucleolus; plasma membrane Molecular Function:actin filament binding; actin-dependent ATPase activity; motor activity; phosphatidylinositol-3,4,5-triphosphate binding; plus-end directed microfilament motor activity; protein binding; spectrin binding Biological Process: cytoskeleton-dependent intracellular transport; regulation of cell shape; regulation of filopodium formation
NCBI Summary:	This gene encodes a member of the myosin superfamily. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-10 (MYH10). Unconventional myosins contain the basic domains of conventional myosins and are further distinguished from class members by their tail domains. This gene functions as an actin-based molecular motor and plays a role in integration of F-actin and microtubule cytoskeletons during meiosis. [provided by RefSeq, Dec 2011]
UniProt Code:	Q9HD67
NCBI GenInfo Identifier:	205371854
NCBI Gene ID:	4651
NCBI Accession:	Q9HD67.3
UniProt Secondary Accession:	Q9HD67,O94893, Q8IVX5, Q9NYM7, Q9P110, Q9P111, Q9UHF6 A7E2D1,
UniProt Related Accession:	Q9HD67
Molecular Weight:	237kDa
NCBI Full Name:	Unconventional myosin-X
NCBI Synonym Full Names:	myosin X
NCBI Official Symbol:	MYO10
NCBI Protein Information:	unconventional myosin-X
UniProt Protein Name:	Unconventional myosin-X
UniProt Synonym Protein Names:	Unconventional myosin-10
Protein Family:	Unconventional myosin
UniProt Gene Name:	MYO10

	Western blot analysis of extracts of various cell lines, using MYO10 antibody (CAB12471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded mouse brain using MYO10 antibody (CAB12471) (40x lens).
	Immunofluorescence analysis of U2OS cells using MYO10 antibody (CAB12471) at dilution of 1:350. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of A-549 cells using MYO10 antibody (CAB12471) at dilution of 1:350. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using MYO10 antibody (CAB12471) at dilution of 1:350. Blue: DAPI for nuclear staining.