Signal Transduction Antibodies 1
Anti-MYO10 Antibody (CAB12466)
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- SKU:
- CAB12466
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-MYO10 Antibody |
| Antibody SKU: | CAB12466 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 845-944 of human MYO10 (NP_036466.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, A-431, BT-474, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 845-944 of human MYO10 (NP_036466.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EAEL RAQQ EEET RKQQ ELEA LQKS QKEA ELTR ELEK QKEN KQVE EILR LEKE IEDL QRMK EQQE LSLT EASL QKLQ ERRD QELR RLEE EACR AAQE FLES |
| Gene ID: | 4651 |
| Uniprot: | Q9HD67 |
| Cellular Location: | Cell projection, Cytoplasm, Peripheral membrane protein, cell cortex, cytoskeleton, cytosol, filopodium membrane, filopodium tip, lamellipodium, ruffle |
| Calculated MW: | 11kDa/163kDa/237kDa |
| Observed MW: | 270kDa |
| Synonyms: | MYO10, myosin X |
| Background: | This gene encodes a member of the myosin superfamily. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-10 (MYH10). Unconventional myosins contain the basic domains of conventional myosins and are further distinguished from class members by their tail domains. This gene functions as an actin-based molecular motor and plays a role in integration of F-actin and microtubule cytoskeletons during meiosis. |
| UniProt Protein Function: | MYO10: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. MYO10 binds to actin filaments and actin bundles and functions as plus end-directed motor. The tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate or integrins, and mediates cargo transport along actin filaments. Regulates cell shape, cell spreading and cell adhesion. Stimulates the formation and elongation of filopodia. May play a role in neurite outgrowth and axon guidance. Plays a role in formation of the podosome belt in osteoclasts. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 5p15.1 Cellular Component: cytosol; filopodium tip; nucleolus; plasma membrane Molecular Function:actin filament binding; actin-dependent ATPase activity; motor activity; phosphatidylinositol-3,4,5-triphosphate binding; plus-end directed microfilament motor activity; protein binding; spectrin binding Biological Process: cytoskeleton-dependent intracellular transport; regulation of cell shape; regulation of filopodium formation |
| NCBI Summary: | This gene encodes a member of the myosin superfamily. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-10 (MYH10). Unconventional myosins contain the basic domains of conventional myosins and are further distinguished from class members by their tail domains. This gene functions as an actin-based molecular motor and plays a role in integration of F-actin and microtubule cytoskeletons during meiosis. [provided by RefSeq, Dec 2011] |
| UniProt Code: | Q9HD67 |
| NCBI GenInfo Identifier: | 205371854 |
| NCBI Gene ID: | 4651 |
| NCBI Accession: | Q9HD67.3 |
| UniProt Secondary Accession: | Q9HD67,O94893, Q8IVX5, Q9NYM7, Q9P110, Q9P111, Q9UHF6 A7E2D1, |
| UniProt Related Accession: | Q9HD67 |
| Molecular Weight: | 237kDa |
| NCBI Full Name: | Unconventional myosin-X |
| NCBI Synonym Full Names: | myosin X |
| NCBI Official Symbol: | MYO10 |
| NCBI Protein Information: | unconventional myosin-X |
| UniProt Protein Name: | Unconventional myosin-X |
| UniProt Synonym Protein Names: | Unconventional myosin-10 |
| Protein Family: | Unconventional myosin |
| UniProt Gene Name: | MYO10 |
 | Western blot analysis of extracts of various cell lines, using MYO10 antibody (CAB12466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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