Cell Biology Antibodies 4
Anti-MYLK2 Antibody (CAB14295)
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- SKU:
- CAB14295
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MYLK2 Antibody |
| Antibody SKU: | CAB14295 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human MYLK2 (NP_149109.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human MYLK2 (NP_149109.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MATE NGAV ELGI QNPS TDKA PKGP TGER PLAA GKDP GPPD PKKA PDPP TLKK DAKA PASE KGDG TLAQ PSTS SQGP KGEG DRGG GPAE GS |
| Gene ID: | 85366 |
| Uniprot: | Q9H1R3 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 64kDa |
| Observed MW: | 65kDa |
| Synonyms: | MYLK2, KMLC, MLCK, MLCK2, skMLCK |
| Background: | This gene encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle. |
| UniProt Protein Function: | skMLCK: a calcium/calmodulin dependent kinase of the MLCK family. A myosin light chain kinase that is exclusively expressed in adult skeletal muscle. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.18; Protein kinase, CAMK; CAMK group; MLCK family Chromosomal Location of Human Ortholog: 20q13.31 Cellular Component: sarcomere; cytoplasm; nucleus Molecular Function:calmodulin binding; protein binding; calmodulin-dependent protein kinase activity; ATP binding; myosin light chain kinase activity Biological Process: striated muscle contraction; regulation of muscle filament sliding; protein amino acid autophosphorylation; cardiac muscle morphogensis; satellite cell differentiation; peptidyl-threonine phosphorylation; neuromuscular synaptic transmission; cardiac muscle contraction Disease: Cardiomyopathy, Familial Hypertrophic, 1 |
| NCBI Summary: | This gene encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H1R3 |
| NCBI GenInfo Identifier: | 14993776 |
| NCBI Gene ID: | 85366 |
| NCBI Accession: | NP_149109.1 |
| UniProt Secondary Accession: | Q9H1R3,Q569L1, Q96I84, |
| UniProt Related Accession: | Q9H1R3 |
| Molecular Weight: | 64,685 Da |
| NCBI Full Name: | myosin light chain kinase 2, skeletal/cardiac muscle |
| NCBI Synonym Full Names: | myosin light chain kinase 2 |
| NCBI Official Symbol: | MYLK2 |
| NCBI Official Synonym Symbols: | KMLC; MLCK; MLCK2; skMLCK |
| NCBI Protein Information: | myosin light chain kinase 2, skeletal/cardiac muscle |
| UniProt Protein Name: | Myosin light chain kinase 2, skeletal/cardiac muscle |
| Protein Family: | Myosin light chain kinase |
| UniProt Gene Name: | MYLK2 |
| UniProt Entry Name: | MYLK2_HUMAN |
 | Western blot analysis of extracts of Jurkat cells, using MYLK2 antibody (CAB14295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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