Cell Biology Antibodies 4

Anti-MYLK2 Antibody (CAB14295)

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SKU:
CAB14295
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MYLK2 Antibody
Antibody SKU:CAB14295
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human MYLK2 (NP_149109.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human MYLK2 (NP_149109.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATE NGAV ELGI QNPS TDKA PKGP TGER PLAA GKDP GPPD PKKA PDPP TLKK DAKA PASE KGDG TLAQ PSTS SQGP KGEG DRGG GPAE GS
Gene ID:85366
Uniprot:Q9H1R3
Cellular Location:Cytoplasm
Calculated MW:64kDa
Observed MW:65kDa
Synonyms:MYLK2, KMLC, MLCK, MLCK2, skMLCK
Background:This gene encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle.
UniProt Protein Function:skMLCK: a calcium/calmodulin dependent kinase of the MLCK family. A myosin light chain kinase that is exclusively expressed in adult skeletal muscle.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.18; Protein kinase, CAMK; CAMK group; MLCK family

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: sarcomere; cytoplasm; nucleus

Molecular Function:calmodulin binding; protein binding; calmodulin-dependent protein kinase activity; ATP binding; myosin light chain kinase activity

Biological Process: striated muscle contraction; regulation of muscle filament sliding; protein amino acid autophosphorylation; cardiac muscle morphogensis; satellite cell differentiation; peptidyl-threonine phosphorylation; neuromuscular synaptic transmission; cardiac muscle contraction

Disease: Cardiomyopathy, Familial Hypertrophic, 1

NCBI Summary:This gene encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle. [provided by RefSeq, Jul 2008]
UniProt Code:Q9H1R3
NCBI GenInfo Identifier:14993776
NCBI Gene ID:85366
NCBI Accession:NP_149109.1
UniProt Secondary Accession:Q9H1R3,Q569L1, Q96I84,
UniProt Related Accession:Q9H1R3
Molecular Weight:64,685 Da
NCBI Full Name:myosin light chain kinase 2, skeletal/cardiac muscle
NCBI Synonym Full Names:myosin light chain kinase 2
NCBI Official Symbol:MYLK2  
NCBI Official Synonym Symbols:KMLC; MLCK; MLCK2; skMLCK  
NCBI Protein Information:myosin light chain kinase 2, skeletal/cardiac muscle
UniProt Protein Name:Myosin light chain kinase 2, skeletal/cardiac muscle
Protein Family:Myosin light chain kinase
UniProt Gene Name:MYLK2  
UniProt Entry Name:MYLK2_HUMAN
Anti-MYLK2 Antibody (CAB14295)Western blot analysis of extracts of Jurkat cells, using MYLK2 antibody (CAB14295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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