Name: Anti-MYLIP Antibody (CAB6166)
Brand: Signal Transduction Antibodies 3
SKU: CAB6166
Availability: OutOfStock

Antibody Name:	Anti-MYLIP Antibody
Antibody SKU:	CAB6166
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 266-445 of human MYLIP (NP_037394.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, Mouse kidney, Mouse liver, Mouse spleen, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 266-445 of human MYLIP (NP_037394.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RAIT ETHA FYRC DTVT SAVM MQYS RDLK GHLA SLFL NENI NLGK KYVF DIKR TSKE VYDH ARRA LYNA GVVD LVSR NNQS PSHS PLKS SESS MNCS SCEG LSCQ QTRV LQEK LRKL KEAM LCMV CCEE EINS TFCP CGHT VCCE SCAA QLQS CPVC RSRV EHVQ HVYL PTHT SLLN LTVI
Gene ID:	29116
Uniprot:	Q8WY64
Cellular Location:	Cell membrane, Cytoplasm, Peripheral membrane protein
Calculated MW:	14kDa/49kDa
Observed MW:	50kDa

Synonyms:	MYLIP, IDOL, MIR
Background:	The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth.

UniProt Protein Function:	MYLIP: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ligase; Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Motility/polarity/chemotaxis; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 6p22.3 Cellular Component: extrinsic to membrane; cytoskeleton; cytoplasm; plasma membrane; intracellular Molecular Function:protein binding; zinc ion binding; cytoskeletal protein binding; ubiquitin-protein ligase activity; ligase activity Biological Process: nervous system development; cholesterol homeostasis; protein destabilization; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; protein ubiquitination; regulation of low-density lipoprotein receptor catabolic process; cell motility
NCBI Summary:	The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8WY64
NCBI GenInfo Identifier:	38788243
NCBI Gene ID:	29116
NCBI Accession:	NP_037394.2
UniProt Secondary Accession:	Q8WY64,Q5TIA4, Q9BU73, Q9NRL9, Q9UHE7,
UniProt Related Accession:	Q8WY64
Molecular Weight:	49,910 Da
NCBI Full Name:	E3 ubiquitin-protein ligase MYLIP
NCBI Synonym Full Names:	myosin regulatory light chain interacting protein
NCBI Official Symbol:	MYLIP
NCBI Official Synonym Symbols:	MIR; IDOL
NCBI Protein Information:	E3 ubiquitin-protein ligase MYLIP; band 4.1 superfamily member BZF1; inducible degrader of the LDL-receptor; cellular modulator of immune recognition (c-MIR); myosin regulatory light chain-interacting protein; E3 ubiquitin ligase-inducible degrader of the
UniProt Protein Name:	E3 ubiquitin-protein ligase MYLIP
UniProt Synonym Protein Names:	Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain interacting protein
UniProt Gene Name:	MYLIP
UniProt Entry Name:	MYLIP_HUMAN

	Western blot analysis of extracts of various cell lines, using MYLIP antibody (CAB6166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human prostate using MYLIP antibody (CAB6166) at dilution of 1:100 (40x lens).