Cell Biology Antibodies 10
Anti-MYL3 Antibody (CAB6662)
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- SKU:
- CAB6662
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MYL3 Antibody |
| Antibody SKU: | CAB6662 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human MYL3 (NP_000249.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Mouse skeletal muscle, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human MYL3 (NP_000249.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAPK KPEP KKDD AKAA PKAA PAPA PPPE PERP KEVE FDAS KIKI EFTP EQIE EFKE AFML FDRT PKCE MKIT YGQC GDVL RALG QNPT QAEV LRVL GKPR QEEL NTKM MDFE TFLP MLQH ISKN KDTG TYED FVEG LRVF DKEG NGTV MGAE LRHV LATL GERL TEDE VEKL MAGQ EDSN GCIN YEAF VKHI MSS |
| Gene ID: | 4634 |
| Uniprot: | P08590 |
| Cellular Location: | |
| Calculated MW: | 21kDa |
| Observed MW: | 22kDa |
| Synonyms: | MYL3, CMH8, MLC-lV/sb, MLC1SB, MLC1V, VLC1, VLCl |
| Background: | MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. |
| UniProt Protein Function: | MYL3: Regulatory light chain of myosin. Does not bind calcium. Defects in MYL3 are the cause of familial hypertrophic cardiomyopathy type 8 (CMH8). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 3p21.3-p21.2 Cellular Component: I band; sarcomere; muscle myosin complex; cytosol; A band Molecular Function:actin monomer binding; structural constituent of muscle; motor activity; myosin II heavy chain binding; calcium ion binding Biological Process: skeletal muscle development; metabolic process; positive regulation of ATPase activity; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; regulation of the force of heart contraction; muscle filament sliding; cardiac muscle contraction Disease: Cardiomyopathy, Familial Hypertrophic, 8 |
| NCBI Summary: | MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P08590 |
| NCBI GenInfo Identifier: | 127149 |
| NCBI Gene ID: | 4634 |
| NCBI Accession: | P08590.3 |
| UniProt Secondary Accession: | P08590,Q9NRS8, B2R534, |
| UniProt Related Accession: | P08590 |
| Molecular Weight: | 195 |
| NCBI Full Name: | Myosin light chain 3 |
| NCBI Synonym Full Names: | myosin, light chain 3, alkali; ventricular, skeletal, slow |
| NCBI Official Symbol: | MYL3 |
| NCBI Official Synonym Symbols: | CMH8; VLC1; MLC1V; MLC1SB |
| NCBI Protein Information: | myosin light chain 3; CMLC1; cardiac myosin light chain 1; ventricular/slow twitch myosin alkali light chain; myosin light chain 1, slow-twitch muscle B/ventricular isoform; myosin, light polypeptide 3, alkali; ventricular, skeletal, slow |
| UniProt Protein Name: | Myosin light chain 3 |
| UniProt Synonym Protein Names: | Cardiac myosin light chain 1; CMLC1; Myosin light chain 1, slow-twitch muscle B/ventricular isoform; MLC1SB; Ventricular/slow twitch myosin alkali light chain |
| Protein Family: | Myosin |
| UniProt Gene Name: | MYL3 |
| UniProt Entry Name: | MYL3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYL3 antibody (CAB6662) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunofluorescence analysis of U2OS cells using MYL3 antibody (CAB6662). Blue: DAPI for nuclear staining. |
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