Name: Anti-MYL2 Antibody (CAB5473)
Brand: Cell Biology Antibodies 9
SKU: CAB5473
Availability: OutOfStock

Antibody Name:	Anti-MYL2 Antibody
Antibody SKU:	CAB5473
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MYL2 (NP_000423.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MYL2 (NP_000423.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPK KAKK RAGG ANSN VFSM FEQT QIQE FKEA FTIM DQNR DGFI DKND LRDT FAAL GRVN VKNE EIDE MIKE APGP INFT VFLT MFGE KLKG ADPE ETIL NAFK VFDP EGKG VLKA DYVR EMLT TQAE RFSK EEVD QMFA AFPP DVTG NLDY KNLV HIIT HGEE KD
Gene ID:	4633
Uniprot:	P10916
Cellular Location:	A band, Cytoplasm, myofibril, sarcomere
Calculated MW:	18kDa
Observed MW:	16kDa

Synonyms:	MYL2, CMH10, MLC-2s/v, MLC2
Background:	Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

UniProt Protein Function:	MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
UniProt Protein Details:	Protein type:Contractile Chromosomal Location of Human Ortholog: 12q24.11 Cellular Component: sarcomere; cytoskeleton; myofibril; cytosol; myosin complex; A band; actin cytoskeleton Molecular Function:actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; muscle filament sliding; cardiac muscle contraction; post-embryonic development Disease: Cardiomyopathy, Familial Hypertrophic, 10
NCBI Summary:	Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
UniProt Code:	P10916
NCBI GenInfo Identifier:	6166556
NCBI Gene ID:	4633
NCBI Accession:	P10916.3
UniProt Secondary Accession:	P10916,Q16123,
UniProt Related Accession:	P10916
Molecular Weight:	166
NCBI Full Name:	Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
NCBI Synonym Full Names:	myosin, light chain 2, regulatory, cardiac, slow
NCBI Official Symbol:	MYL2
NCBI Official Synonym Symbols:	MLC2; CMH10
NCBI Protein Information:	myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, light polypeptide 2, regulatory, cardiac, slow
UniProt Protein Name:	Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Protein Family:	Myosin regulatory
UniProt Gene Name:	MYL2
UniProt Entry Name:	MLRV_HUMAN

	Western blot analysis of extracts of various cell lines, using MYL2 antibody (CAB5473) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of rat heart using MYL2 Rabbit pAb (CAB5473) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse heart using MYL2 Rabbit pAb (CAB5473) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.