Cell Biology Antibodies 12
Anti-MYL1 Antibody (CAB8438)
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- SKU:
- CAB8438
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MYL1 Antibody |
| Antibody SKU: | CAB8438 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human MYL1 (NP_524146.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, Mouse heart, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human MYL1 (NP_524146.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSFS ADQI AEFK EAFL LFDR TGDS KITL SQVG DVLR ALGT NPTN AEVR KVLG NPSN EELN AKKI EFEQ FLPM MQAI SNNK DQAT YEDF VEGL RVFD KEGN GTVM GAEL RHVL ATLG EKMK EEEV EALM AGQE DSNG CINY EAFV KHIM SI |
| Gene ID: | 4632 |
| Uniprot: | P05976 |
| Cellular Location: | |
| Calculated MW: | 16kDa/21kDa |
| Observed MW: | 27kDa |
| Synonyms: | MYL1, MLC1F, MLC3F |
| Background: | Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. |
| UniProt Protein Function: | MYL1: Regulatory light chain of myosin. Does not bind calcium. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Contractile Chromosomal Location of Human Ortholog: 2q33-q34 Cellular Component: sarcomere; myofibril; muscle myosin complex; cytosol Molecular Function:structural constituent of muscle; calcium ion binding Biological Process: muscle contraction; muscle filament sliding; cardiac muscle contraction |
| NCBI Summary: | Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P05976 |
| NCBI GenInfo Identifier: | 17986273 |
| NCBI Gene ID: | 4632 |
| NCBI Accession: | NP_524144.1 |
| UniProt Secondary Accession: | P05976,P06741, Q6IBD5, B2R4N6, B2R4T6, |
| UniProt Related Accession: | P05976 |
| Molecular Weight: | 16,684 Da |
| NCBI Full Name: | myosin light chain 1/3, skeletal muscle isoform isoform 1f |
| NCBI Synonym Full Names: | myosin, light chain 1, alkali; skeletal, fast |
| NCBI Official Symbol: | MYL1 |
| NCBI Official Synonym Symbols: | MLC1F; MLC3F |
| NCBI Protein Information: | myosin light chain 1/3, skeletal muscle isoform; A1 catalytic; A2 catalytic; MLC1/MLC3; MLC1F/MLC3F; myosin light chain A1/A2; myosin light chain alkali 1/2; myosin, light polypeptide 1, alkali; skeletal, fast |
| UniProt Protein Name: | Myosin light chain 1/3, skeletal muscle isoform |
| UniProt Synonym Protein Names: | Myosin light chain alkali 1/2; Myosin light chain A1/A2 |
| Protein Family: | Myosin |
| UniProt Gene Name: | MYL1 |
| UniProt Entry Name: | MYL1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYL1 antibody (CAB8438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded human prostate using MYL1 antibody (CAB8438) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using MYL1 antibody (CAB8438) at dilution of 1:100 (40x lens). |
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