Cell Biology Antibodies 5
Anti-MYH4 Antibody (CAB15293)
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- SKU:
- CAB15293
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MYH4 Antibody |
| Antibody SKU: | CAB15293 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1450-1550 of human MYH4 (NP_060003.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse skeletal muscle, Rat skeletal muscle |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1450-1550 of human MYH4 (NP_060003.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QRNF DKVL AEWK QKYE ETQA ELEA SQKE SRSL STEL FKVK NAYE ESLD HLET LKRE NKNL QQEI SDLT EQIA EGGK HIHE LEKV KKQL DHEK SELQ TSLE E |
| Gene ID: | 4622 |
| Uniprot: | Q9Y623 |
| Cellular Location: | Cytoplasm, myofibril |
| Calculated MW: | |
| Observed MW: | 223kDa |
| Synonyms: | MYH4, MYH2B, MyHC-2B, MyHC-IIb, myosin-4 |
| Background: |
| UniProt Protein Function: | MYH4: Muscle contraction. |
| UniProt Protein Details: | Protein type:Motor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: sarcomere; myofibril; muscle myosin complex Molecular Function:microfilament motor activity; calmodulin binding; double-stranded RNA binding; actin binding; ATP binding Biological Process: muscle contraction; metabolic process; actin filament-based movement; response to activity; muscle filament sliding |
| UniProt Code: | Q9Y623 |
| NCBI GenInfo Identifier: | 224471840 |
| NCBI Gene ID: | 4622 |
| NCBI Accession: | Q9Y623.2 |
| UniProt Related Accession: | Q9Y623 |
| Molecular Weight: | 223,071 Da |
| NCBI Full Name: | Myosin-4 |
| NCBI Synonym Full Names: | myosin, heavy chain 4, skeletal muscle |
| NCBI Official Symbol: | MYH4 |
| NCBI Official Synonym Symbols: | MYH2B; MyHC-2B; MyHC-IIb |
| NCBI Protein Information: | myosin-4; myosin heavy chain 4; myosin heavy chain 2b; myosin heavy chain IIb; myosin heavy chain, skeletal muscle, fetal; myosin, heavy polypeptide 4, skeletal muscle |
| UniProt Protein Name: | Myosin-4 |
| UniProt Synonym Protein Names: | Myosin heavy chain 2b; MyHC-2b; Myosin heavy chain 4; Myosin heavy chain IIb; MyHC-IIb; Myosin heavy chain, skeletal muscle, fetal |
| Protein Family: | Myosin |
| UniProt Gene Name: | MYH4 |
| UniProt Entry Name: | MYH4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYH4 antibody (CAB15293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunofluorescence analysis of mouse skeletal muscle using MYH4 antibody (CAB15293) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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