Cell Biology Antibodies 5
Anti-MYH13 Antibody (CAB14620)
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- SKU:
- CAB14620
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MYH13 Antibody |
| Antibody SKU: | CAB14620 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1240-1320 of human MYH13 (NP_003793.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse eye, Rat eye |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1240-1320 of human MYH13 (NP_003793.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EALS KSKS NIER TCRT VEDQ FSEI KAKD EQQT QLIH DLNM QKAR LQTQ NGEL SHRV EEKE SLIS QLTK SKQA LTQQ LEEL K |
| Gene ID: | 8735 |
| Uniprot: | Q9UKX3 |
| Cellular Location: | Cytoplasm, myofibril |
| Calculated MW: | 223kDa |
| Observed MW: | 250kDa |
| Synonyms: | MYH13, MyHC-IIL, MyHC-eo, myosin-13 |
| Background: |
| UniProt Protein Function: | MYH4: Muscle contraction. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: myofibril Molecular Function:double-stranded RNA binding Biological Process: muscle contraction |
| UniProt Code: | Q9UKX3 |
| NCBI GenInfo Identifier: | 224471840 |
| NCBI Gene ID: | 4622 |
| NCBI Accession: | Q9Y623.2 |
| UniProt Secondary Accession: | Q9UKX3,Q076A3, Q9UKX3, Q9BE40, Q076A6, Q8MJV0, Q5SX40 Q9TV61, P02565, Q076A5, Q9TV62, Q28641, Q29RW1, P13539 Q02566 |
| UniProt Related Accession: | Q9Y623 |
| Molecular Weight: | 223,071 Da |
| NCBI Full Name: | Myosin-4 |
| NCBI Synonym Full Names: | myosin heavy chain 4 |
| NCBI Official Symbol: | MYH4 |
| NCBI Official Synonym Symbols: | MYH2B; MyHC-2B; MyHC-IIb |
| NCBI Protein Information: | myosin-4 |
| UniProt Protein Name: | Myosin-4 |
| UniProt Synonym Protein Names: | Myosin heavy chain 2b; MyHC-2b; Myosin heavy chain 4; Myosin heavy chain IIb; MyHC-IIb; Myosin heavy chain, skeletal muscle, fetal |
| Protein Family: | Myosin |
| UniProt Gene Name: | MYH4 |
| UniProt Entry Name: | MYH4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYH13 antibody (CAB14620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of rat eye using MYH13 Polyclonal Antibody (CAB14620) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse eye using MYH13 Polyclonal Antibody (CAB14620) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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