Cell Biology Antibodies 2

Anti-MYH10 Antibody (CAB12029)

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SKU:
CAB12029
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MYH10 Antibody
Antibody SKU:CAB12029
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1908-2007 of human MYH10 (NP_001242941.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, NIH/3T3, HT-29, Mouse brain, Mouse kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1908-2007 of human MYH10 (NP_001242941.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ARMK QLKR QLEE AEEE ATRA NASR RKLQ RELD DATE ANEG LSRE VSTL KNRL RRGG PISF SSSR SGRR QLHL EGAS LELS DDDT ESKT SDVN ETQP PQSE
Gene ID:4628
Uniprot:P35580
Cellular Location:Cell projection, lamellipodium
Calculated MW:228kDa/230kDa/231kDa/232kDa
Observed MW:250kDa
Synonyms:MYH10, NMMHC-IIB, NMMHCB, myosin-10
Background:This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:MYH10: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with PLEKHG6. Interacts with ECM29. Interacts with KIF26B. Interacts with LARP6. Isoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: mitochondrion; myosin II complex; dendritic spine; actomyosin; cell cortex; actin cytoskeleton; growth cone; cell soma; axon; lamellipodium; cytoplasm; plasma membrane; stress fiber; spindle; midbody; neuromuscular junction; nucleus; myosin complex; cleavage furrow

Molecular Function:calmodulin binding; microfilament motor activity; actin filament binding; protein binding; ADP binding; actin-dependent ATPase activity; actin binding; ATP binding

Biological Process: adult heart development; axon guidance; exocytosis; metabolic process; actin filament-based movement; in utero embryonic development; fourth ventricle development; ventricular cardiac muscle cell development; actomyosin structure organization and biogenesis; cerebellar Purkinje cell layer development; neuron migration; cardiac myofibril assembly; third ventricle development; substrate-bound cell migration, cell extension; regulation of cell shape; cell proliferation; cytokinesis after mitosis; plasma membrane repair; retina development in camera-type eye; ephrin receptor signaling pathway; neuromuscular process controlling balance; cell adhesion; nuclear migration; lateral ventricle development

NCBI Summary:This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:P35580
NCBI GenInfo Identifier:215274129
NCBI Gene ID:4628
NCBI Accession:P35580.3
UniProt Related Accession:P35580
Molecular Weight:250kDa
NCBI Full Name:Myosin-10
NCBI Synonym Full Names:myosin heavy chain 10
NCBI Official Symbol:MYH10  
NCBI Official Synonym Symbols:NMMHCB; NMMHC-IIB  
NCBI Protein Information:myosin-10
UniProt Protein Name:Myosin-10
UniProt Synonym Protein Names:Cellular myosin heavy chain, type B; Myosin heavy chain 10; Myosin heavy chain, non-muscle IIb; Non-muscle myosin heavy chain B; NMMHC-B; Non-muscle myosin heavy chain IIb; NMMHC II-b; NMMHC-IIB
Protein Family:Myosin
UniProt Gene Name:MYH10  
UniProt Entry Name:MYH10_HUMAN
Anti-MYH10 Antibody (CAB12029)Western blot analysis of extracts of various cell lines, using MYH10 antibody (CAB12029) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-MYH10 Antibody (CAB12029)Immunohistochemistry of paraffin-embedded rat brain using MYH10 antibody (CAB12029) at dilution of 1:100 (40x lens).
Anti-MYH10 Antibody (CAB12029)Immunohistochemistry of paraffin-embedded mouse brain using MYH10 antibody (CAB12029) at dilution of 1:100 (40x lens).
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