Cell Biology Antibodies 10
Anti-MYH1 Antibody (CAB6935)
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- SKU:
- CAB6935
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MYH1 Antibody |
| Antibody SKU: | CAB6935 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human MYH1 (NP_005954.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse skeletal muscle, Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human MYH1 (NP_005954.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSSD SEMA IFGE AAPF LRKS ERER IEAQ NKPF DAKT SVFV VDPK ESFV KATV QSRE GGKV TAKT EAGA TVTV KDDQ VFPM NPPK YDKI EDMA MMTH LHEP |
| Gene ID: | 4619 |
| Uniprot: | P12882 |
| Cellular Location: | Cytoplasm, myofibril |
| Calculated MW: | 223kDa |
| Observed MW: | 250KDa |
| Synonyms: | MYH1, HEL71, MYHSA1, MYHa, MyHC-2X/D, MyHC-2x, myosin-1 |
| Background: | Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. |
| UniProt Protein Function: | MYH1: Muscle contraction. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: muscle myosin complex; A band Molecular Function:calmodulin binding; motor activity; actin binding; ATP binding Biological Process: muscle contraction; metabolic process |
| NCBI Summary: | Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P12882 |
| NCBI GenInfo Identifier: | 226694176 |
| NCBI Gene ID: | 4619 |
| NCBI Accession: | P12882.3 |
| UniProt Secondary Accession: | P12882,Q14CA4, Q9Y622, |
| UniProt Related Accession: | P12882 |
| Molecular Weight: | Calculated MW: 223kDaObserved MW: 260kDa |
| NCBI Full Name: | Myosin-1 |
| NCBI Synonym Full Names: | myosin, heavy chain 1, skeletal muscle, adult |
| NCBI Official Symbol: | MYH1 |
| NCBI Official Synonym Symbols: | MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D |
| NCBI Protein Information: | myosin-1; myHC-IIx/d; myosin heavy chain 1; myosin heavy chain 2x; myosin heavy chain IIx/d; epididymis luminal protein 71; myosin heavy chain, skeletal muscle, adult 1; myosin, heavy polypeptide 1, skeletal muscle, adult |
| UniProt Protein Name: | Myosin-1 |
| UniProt Synonym Protein Names: | Myosin heavy chain 1; Myosin heavy chain 2x; MyHC-2x; Myosin heavy chain IIx/d; MyHC-IIx/d; Myosin heavy chain, skeletal muscle, adult 1 |
| UniProt Gene Name: | MYH1 |
| UniProt Entry Name: | MYH1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYH1 antibody (CAB6935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded rat heart using MYH1 Rabbit pAb (CAB6935) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using MYH1 Rabbit pAb (CAB6935) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat heart using MYH1 Rabbit pAb (CAB6935) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse heart using MYH1 Rabbit pAb (CAB6935) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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