Developmental Biology

Anti-MYF5 Antibody (CAB16227)

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SKU:
CAB16227
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-MYF5 Antibody
Antibody SKU:CAB16227
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human MYF5 (NP_005584.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human MYF5 (NP_005584.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDVM DGCQ FSPS EYFY DGSC IPSP EGEF GDEF VPRV AAFG AHKA ELQG SDED EHVR APTG HHQA GHCL MWAC KACK RKST TMDR RKAA TMRE RRRL KKVN QAFE TLKR CTTT NPNQ RLPK VEIL RNAI RYIE SLQE LLRE QVEN YYSL PGQS CSEP TSPT SNCS DGMP ECNS PVWS RKSS TFDS IYCP DVSN VYAT DKNS LSSL DCLS NIVD RITS SEQP GLPL QDLA SLSP VAST DSQP ATPG ASSS RLIY HVL
Gene ID:4617
Uniprot:P13349
Cellular Location:
Calculated MW:28kDa
Observed MW:28kDa
Synonyms:MYF5, bHLHc2
Background:
UniProt Protein Function:Myf-5: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q21

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding

Biological Process: muscle cell fate commitment; muscle development; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; regulation of transcription from RNA polymerase II promoter; skeletal muscle development

UniProt Code:P13349
NCBI GenInfo Identifier:215274028
NCBI Gene ID:4617
NCBI Accession:P13349.2
UniProt Secondary Accession:P13349,Q6ISR9,
UniProt Related Accession:P13349
Molecular Weight:28,296 Da
NCBI Full Name:Myogenic factor 5
NCBI Synonym Full Names:myogenic factor 5
NCBI Official Symbol:MYF5  
NCBI Official Synonym Symbols:bHLHc2  
NCBI Protein Information:myogenic factor 5
UniProt Protein Name:Myogenic factor 5
UniProt Synonym Protein Names:Class C basic helix-loop-helix protein 2; bHLHc2
Protein Family:Myogenic factor
UniProt Gene Name:MYF5  
UniProt Entry Name:MYF5_HUMAN
Anti-MYF5 Antibody (CAB16227)Western blot analysis of extracts of various cell lines, using MYF5 antibody (CAB16227) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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