Epigenetics & Nuclear Signaling Antibodies 2
Anti-MYEF2 Antibody (CAB15829)
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- SKU:
- CAB15829
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-MYEF2 Antibody |
| Antibody SKU: | CAB15829 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human MYEF2 (NP_001288139.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Mouse lung, Mouse testis, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human MYEF2 (NP_001288139.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADA NKAE VPGA TGGD SPHL QPAE PPGE PRRE PHPA EAEK QQPQ HSSS SNGV KMEN DESA KEEK SDLK EKST GSKK ANRF HPYS KDKN SGTG EKKG PNRN RVFI SNIP YDMK WQAI KDLM |
| Gene ID: | 50804 |
| Uniprot: | Q9P2K5 |
| Cellular Location: | Nucleus |
| Calculated MW: | 14kDa/20kDa/61kDa/64kDa |
| Observed MW: | 64kDa |
| Synonyms: | MYEF2, HsT18564, MEF-2, MST156, MSTP156, myEF-2 |
| Background: |
| UniProt Protein Function: | Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA. |
| UniProt Code: | Q9P2K5 |
| NCBI GenInfo Identifier: | 296439294 |
| NCBI Gene ID: | 50804 |
| NCBI Accession: | Q9P2K5.3 |
| UniProt Secondary Accession: | Q9P2K5,Q6NUM5, Q7L388, Q7Z4B7, Q9H922, Q9NUQ1, A7MCZ9 C9J921, C9K0J4, |
| UniProt Related Accession: | Q9P2K5 |
| Molecular Weight: | 14,125 Da |
| NCBI Full Name: | Myelin expression factor 2 |
| NCBI Synonym Full Names: | myelin expression factor 2 |
| NCBI Official Symbol: | MYEF2 |
| NCBI Official Synonym Symbols: | MEF-2; MST156; myEF-2; MSTP156; HsT18564 |
| NCBI Protein Information: | myelin expression factor 2 |
| UniProt Protein Name: | Myelin expression factor 2 |
| UniProt Synonym Protein Names: | MST156 |
| Protein Family: | Myelin expression factor |
| UniProt Gene Name: | MYEF2 |
 | Western blot analysis of extracts of various cell lines, using MYEF2 antibody (CAB15829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded Mouse brain using MYEF2 Rabbit pAb (CAB15829) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat testis using MYEF2 Rabbit pAb (CAB15829) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human liver cancer using MYEF2 Rabbit pAb (CAB15829) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using MYEF2 antibody (CAB15829) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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