Antibody Name:	Anti-MYCN Antibody
Antibody SKU:	CAB0499
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 168-267 of human MYCN (NP_005369.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, Mouse heart, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 168-267 of human MYCN (NP_005369.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AGRA GAAL PAEL AHPA AECV DPAV VFPF PVNK REPA PVPA APAS APAA GPAV ASGA GIAA PAGA PGVA PPRP GGRQ TSGG DHKA LSTS GEDT LSDS DDED
Gene ID:	4613
Uniprot:	P04198
Cellular Location:	Nucleus
Calculated MW:	49kDa
Observed MW:	49kDa

Synonyms:

MODED, N-myc, NMYC, ODED, bHLHe37, MYCN

Background:

This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	N-Myc: a proto-oncogenic transcription factor. Plays a role in cell proliferation, apoptosis and in the development of human tumors.
UniProt Protein Details:	Protein type:Oncoprotein; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 2p24.3 Cellular Component: nucleus; chromatin Molecular Function:protein dimerization activity; protein binding; DNA binding; transcription factor activity Biological Process: regulation of transcription from RNA polymerase II promoter; branching morphogenesis of a tube; positive regulation of mesenchymal cell proliferation; negative regulation of astrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; lung development; cartilage condensation; embryonic skeletal morphogenesis Disease: Feingold Syndrome 1; Tracheoesophageal Fistula With Or Without Esophageal Atresia
NCBI Summary:	This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
UniProt Code:	P04198
NCBI GenInfo Identifier:	127604
NCBI Gene ID:	4613
NCBI Accession:	P04198.2
UniProt Secondary Accession:	P04198,Q53XS5, Q6LDT9,
UniProt Related Accession:	P04198
Molecular Weight:	65kDa
NCBI Full Name:	N-myc proto-oncogene protein
NCBI Synonym Full Names:	v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog
NCBI Official Symbol:	MYCN
NCBI Official Synonym Symbols:	NMYC; ODED; MODED; N-myc; bHLHe37
NCBI Protein Information:	N-myc proto-oncogene protein; pp65/67; oncogene NMYC; neuroblastoma MYC oncogene; class E basic helix-loop-helix protein 37; neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene
UniProt Protein Name:	N-myc proto-oncogene protein
UniProt Synonym Protein Names:	Class E basic helix-loop-helix protein 37; bHLHe37
Protein Family:	N-myc protein
UniProt Gene Name:	MYCN
UniProt Entry Name:	MYCN_HUMAN

	Western blot analysis of extracts of various cell lines, using MYCN antibody (CAB0499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using MYCN antibody (CAB0499) (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using MYCN antibody (CAB0499) (40x lens).