Antibody Name:	Anti-MXI1 Antibody
Antibody SKU:	CAB6661
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 76-295 of human MXI1 (NP_569157.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	mouse kidney, rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 76-295 of human MXI1 (NP_569157.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LLEA ASYL EQIE KENK KCEH GYAS SFPS MPSP RLQH SKPP RRLS RAQK HSSG SSNT STAN RSTH NELE KNRR AHLR LCLE RLKV LIPL GPDC TRHT TLGL LNKA KAHI KKLE EAER KSQH QLEN LERE QRFL KWRL EQLQ GPQE MERI RMDS IGST ISSD RSDS EREE IEVD VEST EFSH GEVD NIST TSIS DIDD HSSL PSIG SDEG YSSA SVKL SFTS
Gene ID:	4601
Uniprot:	P50539
Cellular Location:	Nucleus
Calculated MW:	20kDa/21kDa/26kDa/32kDa
Observed MW:	35kDa

Synonyms:

MXI1, MAD2, MXD2, MXI, bHLHc11

Background:

Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally.

UniProt Protein Function:	Mad2: Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX. Defects in MXI1 may be a cause of susceptibility to prostate cancer (PC). It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; Oncoprotein Chromosomal Location of Human Ortholog: 10q24-q25 Cellular Component: cytoplasm; nucleolus; nucleus Molecular Function:protein binding; transcription corepressor activity Biological Process: cytoplasmic sequestering of transcription factor; negative regulation of cell proliferation Disease: Prostate Cancer
NCBI Summary:	Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally. [provided by RefSeq, Jul 2008]
UniProt Code:	P50539
NCBI GenInfo Identifier:	116242666
NCBI Gene ID:	4601
NCBI Accession:	P50539.2
UniProt Secondary Accession:	P50539,Q15887, Q6FHW2, Q96E53, B1ANN7, D3DR25, D3DRA9
UniProt Related Accession:	P50539
Molecular Weight:	20,558 Da
NCBI Full Name:	Max-interacting protein 1
NCBI Synonym Full Names:	MAX interactor 1, dimerization protein
NCBI Official Symbol:	MXI1
NCBI Official Synonym Symbols:	MXI; MAD2; MXD2; bHLHc11
NCBI Protein Information:	max-interacting protein 1
UniProt Protein Name:	Max-interacting protein 1
UniProt Synonym Protein Names:	Class C basic helix-loop-helix protein 11; bHLHc11
Protein Family:	Max-interacting protein
UniProt Gene Name:	MXI1
UniProt Entry Name:	MXI1_HUMAN

	Western blot analysis of extracts of various cell lines, using MXI1 antibody (CAB6661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using MXI1 antibody (CAB6661). Blue: DAPI for nuclear staining.