Epigenetics & Nuclear Signaling Antibodies 4

Anti-MUS81 Antibody (CAB6818)

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SKU:
CAB6818
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-MUS81 Antibody
Antibody SKU:CAB6818
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MUS81 (NP_079404.3).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MUS81 (NP_079404.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAP VRLG RKRP LPAC PNPL FVRW LTEW RDEA TRSR RRTR FVFQ KALR SLRR YPLP LRSG KEAK ILQH FGDG LCRM LDER LQRH RTSG GDHA PDSP SGEN SPAP QGRL AEVQ DSSM PVPA QPKA GGSG SYWP ARHS GARV ILLV LYRE HLNP NGHH FLTK EELL QRCA QKSP RVAP GSAR PWPA LRSL LHRN LVLR THQP ARYS LTPE GLEL AQKL AESE GLSL LNVG IGPK EPPG EETA VPGA ASAE LASE AGVQ QQPL ELRP GEYR VLLC VDIG ETRG GGHR PELL RELQ RLHV THTV RKLH VGDF VWVA QETN PRDP ANPG ELVL DHIV ERKR LDDL CSSI IDGR FR
Gene ID:80198
Uniprot:Q96NY9
Cellular Location:Nucleus, nucleolus
Calculated MW:61kDa
Observed MW:70kDa
Synonyms:MUS81, SLX3
Background:This gene encodes a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks.
UniProt Protein Function:MUS81: Interacts with EME1 and EME2 to form a DNA structure- specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. Belongs to the XPF family.
UniProt Protein Details:

Protein type:Deoxyribonuclease; EC 3.1.22.-; Nucleolus

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleolus; nucleus

Molecular Function:protein binding; DNA binding; 3'-flap endonuclease activity; metal ion binding

Biological Process: DNA repair; DNA catabolic process, endonucleolytic; DNA recombination

UniProt Code:Q96NY9
NCBI GenInfo Identifier:166898077
NCBI Gene ID:80198
NCBI Accession:Q96NY9.3
UniProt Secondary Accession:Q96NY9,Q9H7D9,
UniProt Related Accession:Q96NY9
Molecular Weight:551
NCBI Full Name:Crossover junction endonuclease MUS81
NCBI Synonym Full Names:MUS81 structure-specific endonuclease subunit
NCBI Official Symbol:MUS81  
NCBI Official Synonym Symbols:SLX3  
NCBI Protein Information:crossover junction endonuclease MUS81; MUS81 endonuclease homolog; SLX3 structure-specific endonuclease subunit homolog
UniProt Protein Name:Crossover junction endonuclease MUS81
Protein Family:Crossover junction endonuclease
UniProt Gene Name:MUS81  
UniProt Entry Name:MUS81_HUMAN
Anti-MUS81 Antibody (CAB6818)Western blot analysis of extracts of various cell lines, using MUS81 antibody (CAB6818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-MUS81 Antibody (CAB6818)Immunohistochemistry of paraffin-embedded rat brain using MUS81 antibody (CAB6818) at dilution of 1:100 (40x lens).
Anti-MUS81 Antibody (CAB6818)Immunohistochemistry of paraffin-embedded mouse brain using MUS81 antibody (CAB6818) at dilution of 1:100 (40x lens).
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