Name: Anti-MUC4 Antibody (CAB16922)
Brand: Cell Biology Antibodies 7
SKU: CAB16922
Availability: OutOfStock

Antibody Name:	Anti-MUC4 Antibody
Antibody SKU:	CAB16922
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human MUC4.

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:

Immunogen:	A synthetic peptide of human MUC4.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4585
Uniprot:	Q99102
Cellular Location:
Calculated MW:	112-130kDa/144kDa/194-231kDa
Observed MW:	Refer to figures

Synonyms:

MUC4, ASGP, HSA276359, MUC-4, mucin-4

Background:

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats.

UniProt Protein Function:	MUC4: May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. 14 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 3q29 Cellular Component: extracellular space; Golgi lumen; integral to plasma membrane; vesicle Molecular Function:ErbB-2 class receptor binding Biological Process: maintenance of gastrointestinal epithelium; O-glycan processing
NCBI Summary:	The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99102
NCBI GenInfo Identifier:	338817990
NCBI Gene ID:	4585
NCBI Accession:	Q99102.4
UniProt Secondary Accession:	Q99102,O95938, Q9GZM2, Q9GZV6, Q9H481, Q9H482, Q9H483 Q9H484, Q9H485, Q9H486, Q9H487, Q9H4D6,
UniProt Related Accession:	Q99102
Molecular Weight:	206,605 Da
NCBI Full Name:	Mucin-4
NCBI Synonym Full Names:	mucin 4, cell surface associated
NCBI Official Symbol:	MUC4
NCBI Official Synonym Symbols:	ASGP; MUC-4; HSA276359
NCBI Protein Information:	mucin-4
UniProt Protein Name:	Mucin-4
UniProt Synonym Protein Names:	Ascites sialoglycoprotein; ASGP
Protein Family:	Mucin
UniProt Gene Name:	MUC4

Western blot analysis of extracts of HeLa cells, using MUC4 antibody (CAB16922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.

ELISA Kits

Human Mucin-4 / MUC4 ELISA Kit

Human MUC4 (Mucin 4) CLIA Kit (HUES00453)

Human MUC4 (Mucin 4) ELISA Kit (HUES01866)

Human Mucin-4 (MUC4) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)