Cell Biology Antibodies 6
Anti-MUC20 Antibody (CAB15968)
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- SKU:
- CAB15968
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MUC20 Antibody |
| Antibody SKU: | CAB15968 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MUC20 (NP_689886.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, A-549, DU145, LO2, U-251MG, HT-29, Mouse kidney, Mouse lung, Rat kidney |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MUC20 (NP_689886.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PNFM VLIA TSVE TSAA SGSP EGAG MTTV QTIT GSDP EEAI FDTL CTDD SSEE AKTL TMDI LTLA HTST EAKG LSSE SSAS SDGP HPVI TPSR ASES SASS D |
| Gene ID: | 200958 |
| Uniprot: | Q8N307 |
| Cellular Location: | Apical cell membrane, Basolateral cell membrane, Cell projection, Secreted, microvillus membrane |
| Calculated MW: | 68kDa/71kDa |
| Observed MW: | 80kDa |
| Synonyms: | MUC20, MUC-20, mucin-20 |
| Background: | This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. |
| UniProt Protein Function: | MUC20: May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 3q29 Cellular Component: Golgi lumen; microvillus membrane; apical plasma membrane; basal plasma membrane; extracellular region Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; activation of MAPK activity; hepatocyte growth factor receptor signaling pathway; post-translational protein modification; protein homooligomerization |
| NCBI Summary: | This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. [provided by RefSeq, Apr 2014] |
| UniProt Code: | Q8N307 |
| NCBI GenInfo Identifier: | 317373415 |
| NCBI Gene ID: | 200958 |
| NCBI Accession: | Q8N307.3 |
| UniProt Secondary Accession: | Q8N307,Q6UX97, Q76I83, Q76I85, Q86ST8, Q8NBY6, Q96KA1 Q9P2I8, |
| UniProt Related Accession: | Q8N307 |
| Molecular Weight: | 538 |
| NCBI Full Name: | Mucin-20 |
| NCBI Synonym Full Names: | mucin 20, cell surface associated |
| NCBI Official Symbol: | MUC20 |
| NCBI Official Synonym Symbols: | MUC-20 |
| NCBI Protein Information: | mucin-20; transmembrane mucin MUC20S |
| UniProt Protein Name: | Mucin-20 |
| Protein Family: | Mucin |
| UniProt Gene Name: | MUC20 |
| UniProt Entry Name: | MUC20_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MUC20 antibody (CAB15968) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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