Name: Anti-MTMR14 Antibody (CAB7480)
Brand: Cell Biology Antibodies 11
SKU: CAB7480
Availability: OutOfStock

Antibody Name:	Anti-MTMR14 Antibody
Antibody SKU:	CAB7480
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MTMR14 (NP_001070993.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, Jurkat, Mouse liver, Mouse spleen, Mouse testis, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MTMR14 (NP_001070993.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGA RAAA AAAS AGSS ASSG NQPP QELG LGEL LEEF SRTQ YRAK DGSG TGGS KVER IEKR CLEL FGRD YCFS VIPN TNGD ICGH YPRH IVFL EYES SEKE KDTF ESTV QVSK LQDL IHRS KMAR CRGR FVCP VILF KGKH ICRS ATLA GWGE LYGR SGYN YFFS GGAD DAWA DVED VTEE DCAL RSGD THLF DKVR GYDI KLLR YLSV KYIC DLMV ENKK VKFG MNVT SSEK VDKA QRYA DFTL LSIP YPGC EFFK EYKD RDYM AEGL IFNW KQDY VDAP
Gene ID:	64419
Uniprot:	Q8NCE2
Cellular Location:	Cytoplasm
Calculated MW:	60kDa/66kDa/72kDa
Observed MW:	72kDa

Synonyms:

MTMR14, C3orf29

Background:

This gene encodes a myotubularin-related protein. The encoded protein is a phosphoinositide phosphatase that specifically dephosphorylates phosphatidylinositol 3, 5-biphosphate and phosphatidylinositol 3-phosphate. Mutations in this gene are correlated with autosomal dominant centronuclear myopathy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 18.

UniProt Protein Function:	MTMR14: Lipid phosphatase which efficiently dephosphorylates phosphatidylinositol 3-phosphate (PtdIns3P) and PtdIns(3,5)P2; inactive toward PtdIns4P, PtdIns(3,4)P2, PtdIns(4,5)P2 and PtdIns(3,4,5)P3. Expressed in various tissues, including heart, skeletal muscle, placenta, liver, lung, kidney and pancreas. Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.-; Phosphatase, lipid Chromosomal Location of Human Ortholog: 3p26 Cellular Component: cytosol; perinuclear region of cytoplasm; ruffle Molecular Function:phosphatidylinositol-3-phosphatase activity; protein binding; protein serine/threonine phosphatase activity Biological Process: macroautophagy; phosphatidylinositol biosynthetic process Disease: Myopathy, Centronuclear, 1
NCBI Summary:	This gene encodes a myotubularin-related protein. The encoded protein is a phosphoinositide phosphatase that specifically dephosphorylates phosphatidylinositol 3,5-biphosphate and phosphatidylinositol 3-phosphate. Mutations in this gene are correlated with autosomal dominant centronuclear myopathy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 18.[provided by RefSeq, Apr 2010]
UniProt Code:	Q8NCE2
NCBI GenInfo Identifier:	118568016
NCBI Gene ID:	64419
NCBI Accession:	Q8NCE2.2
UniProt Secondary Accession:	Q8NCE2,Q0JTH5, Q0JU83, Q6PIZ4, Q6QE21, Q86VK9, Q8IYK1 Q8TCM7, Q9H6C0,
UniProt Related Accession:	Q8NCE2
Molecular Weight:	60,035 Da
NCBI Full Name:	Myotubularin-related protein 14
NCBI Synonym Full Names:	myotubularin related protein 14
NCBI Official Symbol:	MTMR14
NCBI Official Synonym Symbols:	C3orf29
NCBI Protein Information:	myotubularin-related protein 14
UniProt Protein Name:	Myotubularin-related protein 14
UniProt Synonym Protein Names:	HCV NS5A-transactivated protein 4 splice variant A-binding protein 1; NS5ATP4ABP1; hJumpy
Protein Family:	Myotubularin-related protein
UniProt Gene Name:	MTMR14
UniProt Entry Name:	MTMRE_HUMAN

Western blot analysis of extracts of various cell lines, using MTMR14 antibody (CAB7480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

MTMR14 Antibody (PACO10645)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)