Metabolism Antibodies 3
Anti-MTIF3 Antibody (CAB8524)
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- SKU:
- CAB8524
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-MTIF3 Antibody |
| Antibody SKU: | CAB8524 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-278 of human MTIF3 (NP_690876.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | A-431, HeLa, HT-29 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-278 of human MTIF3 (NP_690876.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAL FLKR LTLQ TVKS ENSC IRCF GKHI LQKT APAQ LSPI ASAP RLSF LIHA KAFS TAED TQNE GKKT KKNK TAFS NVGR KISQ RVIH LFDE KGND LGNM HRAN VIRL MDER DLRL VQRN TSTE PAEY QLMT GLQI LQER QRLR EMEK ANPK TGPT LRKE LILS SNIG QHDL DTKT KQIQ QWIK KKHL VQIT IKKG KNVD VSEN EMEE IFHQ ILQT MPGI ATFS SRPQ AVQG GKAL MCVL RAFS KNEE KAYK ETQE TQER DTLN KDHG NDKE SNVL HQ |
| Gene ID: | 219402 |
| Uniprot: | Q9H2K0 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 31kDa |
| Observed MW: | 32kDa |
| Synonyms: | MTIF3, IF3mt |
| Background: | This gene encodes a translation initiation factor that is involved in mitochondrial protein synthesis. Polymorphism in this gene is associated with the onset of Parkinson's disease. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5. |
| UniProt Protein Function: | MTIF3: IF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins. Belongs to the IF-3 family. |
| UniProt Protein Details: | Protein type:Translation initiation; Mitochondrial; Translation Chromosomal Location of Human Ortholog: 13q12.2 Cellular Component: mitochondrion Molecular Function:protein binding; ribosomal small subunit binding; translation factor activity, nucleic acid binding; translation initiation factor activity Biological Process: mitochondrial translation; organelle organization and biogenesis; ribosome disassembly |
| NCBI Summary: | This gene encodes a translation initiation factor that is involved in mitochondrial protein synthesis. Polymorphism in this gene is associated with the onset of Parkinson's disease. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q9H2K0 |
| NCBI GenInfo Identifier: | 317373570 |
| NCBI Gene ID: | 219402 |
| NCBI Accession: | Q9H2K0.4 |
| UniProt Secondary Accession: | Q9H2K0,Q05BL8, Q5W0V0, Q86X68, |
| UniProt Related Accession: | Q9H2K0 |
| Molecular Weight: | 31,725 Da |
| NCBI Full Name: | Translation initiation factor IF-3, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial translational initiation factor 3 |
| NCBI Official Symbol: | MTIF3 |
| NCBI Official Synonym Symbols: | IF3mt |
| NCBI Protein Information: | translation initiation factor IF-3, mitochondrial |
| UniProt Protein Name: | Translation initiation factor IF-3, mitochondrial |
| UniProt Gene Name: | MTIF3 |
| UniProt Entry Name: | IF3M_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MTIF3 antibody (CAB8524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using MTIF3 antibody (CAB8524) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human prostate using MTIF3 antibody (CAB8524) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using MTIF3 antibody (CAB8524) at dilution of 1:100 (40x lens). |
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