Metabolism Antibodies 1

Anti-MTIF3 Antibody (CAB12615)

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SKU:
CAB12615
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-MTIF3 Antibody
Antibody SKU:CAB12615
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-278 of human MTIF3 (NP_690876.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, Mouse heart, Mouse liver, Mouse kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-278 of human MTIF3 (NP_690876.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAL FLKR LTLQ TVKS ENSC IRCF GKHI LQKT APAQ LSPI ASAP RLSF LIHA KAFS TAED TQNE GKKT KKNK TAFS NVGR KISQ RVIH LFDE KGND LGNM HRAN VIRL MDER DLRL VQRN TSTE PAEY QLMT GLQI LQER QRLR EMEK ANPK TGPT LRKE LILS SNIG QHDL DTKT KQIQ QWIK KKHL VQIT IKKG KNVD VSEN EMEE IFHQ ILQT MPGI ATFS SRPQ AVQG GKAL MCVL RAFS KNEE KAYK ETQE TQER DTLN KDHG NDKE SNVL HQ
Gene ID:219402
Uniprot:Q9H2K0
Cellular Location:Mitochondrion
Calculated MW:31kDa
Observed MW:32kDa
Synonyms:MTIF3, IF3mt
Background:This gene encodes a translation initiation factor that is involved in mitochondrial protein synthesis. Polymorphism in this gene is associated with the onset of Parkinson's disease. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5.
UniProt Protein Function:MTIF3: IF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins. Belongs to the IF-3 family.
UniProt Protein Details:

Protein type:Translation initiation; Mitochondrial; Translation

Chromosomal Location of Human Ortholog: 13q12.2

Cellular Component: mitochondrion

Molecular Function:protein binding; ribosomal small subunit binding; translation factor activity, nucleic acid binding; translation initiation factor activity

Biological Process: mitochondrial translation; organelle organization and biogenesis; ribosome disassembly

NCBI Summary:This gene encodes a translation initiation factor that is involved in mitochondrial protein synthesis. Polymorphism in this gene is associated with the onset of Parkinson's disease. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5. [provided by RefSeq, Oct 2009]
UniProt Code:Q9H2K0
NCBI GenInfo Identifier:317373570
NCBI Gene ID:219402
NCBI Accession:Q9H2K0.4
UniProt Secondary Accession:Q9H2K0,Q05BL8, Q5W0V0, Q86X68,
UniProt Related Accession:Q9H2K0
Molecular Weight:31,725 Da
NCBI Full Name:Translation initiation factor IF-3, mitochondrial
NCBI Synonym Full Names:mitochondrial translational initiation factor 3
NCBI Official Symbol:MTIF3  
NCBI Official Synonym Symbols:IF3mt  
NCBI Protein Information:translation initiation factor IF-3, mitochondrial
UniProt Protein Name:Translation initiation factor IF-3, mitochondrial
UniProt Gene Name:MTIF3  
UniProt Entry Name:IF3M_HUMAN
Anti-MTIF3 Antibody (CAB12615)Western blot analysis of extracts of various cell lines, using MTIF3 antibody (CAB12615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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