Signal Transduction Antibodies 1
Anti-MTHFS Antibody (CAB12770)
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- SKU:
- CAB12770
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-MTHFS Antibody |
| Antibody SKU: | CAB12770 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-203 of human MTHFS (NP_006432.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, A-549, HepG2, HeLa, Jurkat, Mouse liver, Rat stomach, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-203 of human MTHFS (NP_006432.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAA AVSS AKRS LRGE LKQR LRAM SAEE RLRQ SRVL SQKV IAHS EYQK SKRI SIFL SMQD EIET EEII KDIF QRGK ICFI PRYR FQSN HMDM VRIE SPEE ISLL PKTS WNIP QPGE GDVR EEAL STGG LDLI FMPG LGFD KHGN RLGR GKGY YDAY LKRC LQHQ EVKP YTLA LAFK EQIC LQVP VNEN DMKV DEVL YEDS STA |
| Gene ID: | 10588 |
| Uniprot: | P49914 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 20kDa/23kDa |
| Observed MW: | 23kDa |
| Synonyms: | MTHFS, HsT19268 |
| Background: | The protein encoded by this gene is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5, 10-methenyltetrahydrofolate, a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for this gene. |
| UniProt Protein Function: | Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate. |
| NCBI Summary: | The protein encoded by this gene is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011] |
| UniProt Code: | P49914 |
| NCBI GenInfo Identifier: | 1706921 |
| NCBI Gene ID: | 10588 |
| NCBI Accession: | P49914.2 |
| UniProt Related Accession: | P49914 |
| Molecular Weight: | 20kDa; 23kDa |
| NCBI Full Name: | 5-formyltetrahydrofolate cyclo-ligase |
| NCBI Synonym Full Names: | methenyltetrahydrofolate synthetase |
| NCBI Official Symbol: | MTHFS |
| NCBI Official Synonym Symbols: | NEDMEHM; HsT19268 |
| NCBI Protein Information: | 5-formyltetrahydrofolate cyclo-ligase |
| UniProt Protein Name: | 5-formyltetrahydrofolate cyclo-ligase |
| UniProt Synonym Protein Names: | 5,10-methenyl-tetrahydrofolate synthetase; MTHFS; Methenyl-THF synthetase |
| UniProt Gene Name: | MTHFS |
| UniProt Entry Name: | MTHFS_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MTHFS antibody (CAB12770) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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