Name: Anti-MTHFD1L Antibody (CAB7969)
Brand: Metabolism Antibodies 2
SKU: CAB7969
Availability: OutOfStock

Antibody Name:	MTHFD1L Rabbit Polyclonal Antibody
Antibody SKU:	CAB7969
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 759-978 of human MTHFD1L (NP_056255.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, placenta, Mouse brain, Mouse heart, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 759-978 of human MTHFD1L (NP_056255.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GVPL KKEY TEEN IQLV ADGC CNLQ KQIQ ITQL FGVP VVVA LNVF KTDT RAEI DLVC ELAK RAGA FDAV PCYH WSVG GKGS VDLA RAVR EAAS KRSR FQFL YDVQ VPIV DKIR TIAQ AVYG AKDI ELSP EAQA KIDR YTQQ GFGN LPIC MAKT HLSL SHQP DKKG VPRD FILP ISDV RASI GAGF IYPL VGTM STMP GLPT RPCF YDID LDTE TEQV KGLF
Gene ID:	25902
Uniprot:	Q6UB35
Cellular Location:	Mitochondrion
Calculated MW:	29kDa/105kDa
Observed MW:	106kDa

Synonyms:	MTHFD1L, FTHFSDC1, MTC1THFS, dJ292B18.2
Background:	The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	MTHFD1L: May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 6.3.4.3; Mitochondrial; Cofactor and Vitamin Metabolism - one carbon pool by folate; Ligase Chromosomal Location of Human Ortholog: 6q25.1 Cellular Component: membrane; mitochondrion; cytosol Molecular Function:methylenetetrahydrofolate dehydrogenase (NADP+) activity; protein homodimerization activity; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity; ATP binding Biological Process: folic acid and derivative metabolic process; tetrahydrofolate metabolic process; folic acid and derivative biosynthetic process; formate metabolic process; one-carbon compound metabolic process
NCBI Summary:	The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]
UniProt Code:	Q6UB35
NCBI GenInfo Identifier:	74749360
NCBI Gene ID:	25902
NCBI Accession:	Q6UB35.1
UniProt Related Accession:	Q6UB35
Molecular Weight:	106kDa
NCBI Full Name:	Monofunctional C1-tetrahydrofolate synthase, mitochondrial
NCBI Synonym Full Names:	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like
NCBI Official Symbol:	MTHFD1L
NCBI Official Synonym Symbols:	FTHFSDC1; MTC1THFS; dJ292B18.2
NCBI Protein Information:	monofunctional C1-tetrahydrofolate synthase, mitochondrial
UniProt Protein Name:	Monofunctional C1-tetrahydrofolate synthase, mitochondrial
UniProt Synonym Protein Names:	Formyltetrahydrofolate synthetase
Protein Family:	Monofunctional C1-tetrahydrofolate synthase
UniProt Gene Name:	MTHFD1L
UniProt Entry Name:	C1TM_HUMAN

	Western blot analysis of extracts of various cell lines, using MTHFD1L antibody (CAB7969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using MTHFD1L Rabbit pAb (CAB7969) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using MTHFD1L Rabbit pAb (CAB7969) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using MTHFD1L Rabbit pAb (CAB7969) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of A549 cells using MTHFD1L antibody (CAB7969). Blue: DAPI for nuclear staining.