Antibody Name:	Anti-MTHFD1 Antibody
Antibody SKU:	CAB8661
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MTHFD1 (NP_005947.3).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, LO2, Mouse brain, Mouse kidney, Rat spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MTHFD1 (NP_005947.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPA EILN GKEI SAQI RARL KNQV TQLK EQVP GFTP RLAI LQVG NRDD SNLY INVK LKAA EEIG IKAT HIKL PRTT TESE VMKY ITSL NEDS TVHG FLVQ LPLD SENS INTE EVIN AIAP EKDV DGLT SINA GKLA RGDL NDCF IPCT PKGC LELI KETG VPIA GRHA VVVG RSKI VGAP MHDL LLWN NATV TTCH SKTA HLDE EVNK GDIL VVAT GQPE MVKG EWIK PGAI VIDC GINY VPDD KKPN GRKV VGDV AYDE AKER ASFI TP
Gene ID:	4522
Uniprot:	P11586
Cellular Location:	Cytoplasm
Calculated MW:	101kDa
Observed MW:	115kDa

Synonyms:

MTHFD1, MTHFC, MTHFD

Background:

This gene encodes a protein that possesses three distinct enzymatic activities, 5, 10-methylenetetrahydrofolate dehydrogenase, 5, 10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.

UniProt Protein Function:	MTHFD1: an ubiquitous trifunctional enzyme containing two major domains: a N-terminal part, containing methylene-THF dehydrogenase and methenyl-THF cyclohydrolase activities, and a C-terminal part containing a formyl-THF synthetase domain.
UniProt Protein Details:	Protein type:EC 1.5.1.5; Oxidoreductase; Hydrolase; EC 6.3.4.3; Cofactor and Vitamin Metabolism - one carbon pool by folate; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 3.5.4.9; Ligase Chromosomal Location of Human Ortholog: 14q24 Cellular Component: mitochondrion; membrane; cytosol Molecular Function:methylenetetrahydrofolate dehydrogenase (NADP+) activity; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity; ATP binding; methylenetetrahydrofolate dehydrogenase activity Biological Process: histidine biosynthetic process; vitamin metabolic process; folic acid and derivative biosynthetic process; methionine biosynthetic process; purine nucleotide biosynthetic process; one-carbon compound metabolic process; folic acid metabolic process; water-soluble vitamin metabolic process Disease: Neural Tube Defects, Folate-sensitive
NCBI Summary:	This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008]
UniProt Code:	P11586
NCBI GenInfo Identifier:	38014238
NCBI Gene ID:	4522
NCBI Accession:	AAH01014.2
UniProt Secondary Accession:	P11586,Q86VC9, Q9BVP5, B2R5Y2,
UniProt Related Accession:	P11586
Molecular Weight:	101,559 Da
NCBI Full Name:	MTHFD1 protein, partial
NCBI Synonym Full Names:	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
NCBI Official Symbol:	MTHFD1
NCBI Official Synonym Symbols:	MTHFC; MTHFD
NCBI Protein Information:	C-1-tetrahydrofolate synthase, cytoplasmic; C1-THF synthase; cytoplasmic C-1-tetrahydrofolate synthase; 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase
UniProt Protein Name:	C-1-tetrahydrofolate synthase, cytoplasmic
UniProt Gene Name:	MTHFD1
UniProt Entry Name:	C1TC_HUMAN

	Western blot analysis of extracts of various cell lines, using MTHFD1 antibody (CAB8661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of U-2 OS cells using MTHFD1 Rabbit pAb (CAB8661) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.