Cell Biology Antibodies 3

Anti-MTERFD1 Antibody (CAB12585)

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SKU:
CAB12585
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MTERFD1 Antibody
Antibody SKU:CAB12585
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 69-300 of human MTERFD1 (NP_057026.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, NCI-H460, SKOV3, BT-474, Mouse kidney, Rat fat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 69-300 of human MTERFD1 (NP_057026.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SSQS TSSS SQEN NSAQ SSLL PSMN EQSQ KTQN ISSF DSEL FLEE LDEL PPLS PMQP ISEE EAIQ IIAD PPLP PASF TLRD YVDH SETL QKLV LLGV DLSK IEKH PEAA NLLL RLDF EKDI KQML LFLK DVGI EDNQ LGAF LTKN HAIF SEDL ENLK TRVA YLHS KNFS KADV AQMV RKAP FLLN FSVE RLDN RLGF FQKE LELS VKKT RDLV VRLP RLLT GSLE PVKE NMKV
Gene ID:51001
Uniprot:Q96E29
Cellular Location:Mitochondrion
Calculated MW:34kDa/37kDa/47kDa
Observed MW:48kDa
Synonyms:MTERF3, CGI-12, MTERFD1
Background:
UniProt Protein Function:MTERFD1: Binds promoter DNA and regulates initiation of transcription. Required for normal mitochondrial transcription, and for normal assembly of mitochondrial respiratory complexes. Required for normal mitochondrial function. Belongs to the mTERF family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: mitochondrial outer membrane; mitochondrion; nucleoplasm

Molecular Function:protein binding; rRNA binding; single-stranded RNA binding

Biological Process: developmental process; macroautophagy; negative regulation of transcription, DNA-dependent; ribosome assembly; RNA splicing

UniProt Code:Q96E29
NCBI GenInfo Identifier:74731522
NCBI Gene ID:51001
NCBI Accession:Q96E29.2
UniProt Secondary Accession:Q96E29,Q9Y301, B3KMG6, G3V130,
UniProt Related Accession:Q96E29
Molecular Weight:48kDa
NCBI Full Name:Transcription termination factor 3, mitochondrial
NCBI Synonym Full Names:mitochondrial transcription termination factor 3
NCBI Official Symbol:MTERF3  
NCBI Official Synonym Symbols:CGI-12; MTERFD1  
NCBI Protein Information:transcription termination factor 3, mitochondrial
UniProt Protein Name:Transcription termination factor 3, mitochondrial
UniProt Synonym Protein Names:Mitochondrial transcription termination factor 3; mTERF3; mTERF domain-containing protein 1, mitochondrial
UniProt Gene Name:MTERF3  
Anti-MTERFD1 Antibody (CAB12585)Western blot analysis of extracts of various cell lines, using MTERFD1 antibody (CAB12585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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