Epigenetics & Nuclear Signaling Antibodies 2

Anti-MTERF Antibody (CAB14805)

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SKU:
CAB14805
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-MTERF Antibody
Antibody SKU:CAB14805
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 61-240 of human MTERF (NP_008911.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 61-240 of human MTERF (NP_008911.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KCHN TDSE PLKN EDLL KNLL TMGV DIDM ARKR QPGV FHRM ITNE QDLK MFLL SKGA SKEV IASI ISRY PRAI TRTP ENLS KRWD LWRK IVTS DLEI VNIL ERSP ESFF RSNN NLNL ENNI KFLY SVGL TRKC LCRL LTNA PRTF SNSL DLNK QMVE FLQA AGLS LGHN DPAD FVRK IIFK
Gene ID:7978
Uniprot:Q99551
Cellular Location:Mitochondrion
Calculated MW:45kDa
Observed MW:46kDa
Synonyms:MTERF1, MTERF
Background:This gene encodes a mitochondrial transcription termination factor. This protein participates in attenuating transcription from the mitochondrial genome; this attenuation allows higher levels of expression of 16S ribosomal RNA relative to the tRNA gene downstream. The product of this gene has three leucine zipper motifs bracketed by two basic domains that are all required for DNA binding. There is evidence that, for this protein, the zippers participate in intramolecular interactions that establish the three-dimensional structure required for DNA binding.
UniProt Protein Function:MTERF: Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Probably requires one or more components for termination activity. Belongs to the mTERF family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 7q21.2

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function:protein binding; double-stranded DNA binding

Biological Process: termination of mitochondrial transcription; mitochondrion organization and biogenesis; regulation of transcription, DNA-dependent; organelle organization and biogenesis; gene expression; transcription termination; DNA geometric change; transcription from mitochondrial promoter

NCBI Summary:This gene encodes a mitochondrial transcription termination factor. This protein participates in attenuating transcription from the mitochondrial genome; this attenuation allows higher levels of expression of 16S ribosomal RNA relative to the tRNA gene downstream. The product of this gene has three leucine zipper motifs bracketed by two basic domains that are all required for DNA binding. There is evidence that, for this protein, the zippers participate in intramolecular interactions that establish the three-dimensional structure required for DNA binding. [provided by RefSeq, Jul 2008]
UniProt Code:Q99551
NCBI GenInfo Identifier:5902010
NCBI Gene ID:7978
NCBI Accession:NP_008911.1
UniProt Secondary Accession:Q99551,Q32NF8, Q53H51, Q9BVR7, A4D1E3,
UniProt Related Accession:Q99551
Molecular Weight:45,778 Da
NCBI Full Name:transcription termination factor, mitochondrial
NCBI Synonym Full Names:mitochondrial transcription termination factor
NCBI Official Symbol:MTERF  
NCBI Protein Information:transcription termination factor, mitochondrial; mitochondrial transcription termination factor 1
UniProt Protein Name:Transcription termination factor, mitochondrial
UniProt Synonym Protein Names:Mitochondrial transcription termination factor 1
UniProt Gene Name:MTERF  
UniProt Entry Name:MTERF_HUMAN
Anti-MTERF Antibody (CAB14805)Western blot analysis of extracts of various cell lines, using MTERF1 antibody (CAB14805) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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