Antibody Name:	Anti-MTCH2 Antibody
Antibody SKU:	CAB12934
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-175 of human MTCH2 (NP_055157.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HepG2, HeLa, 293T, HT-29, Mouse small intestine, Mouse brain, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-175 of human MTCH2 (NP_055157.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VGYE PLPP TIGR NIFG RQVC QLPG LFSY AQHI ASID GRRG LFTG LTPR LCSG VLGT VVHG KVLQ HYQE SDKG EELG PGNV QKEV SSSF DHVI KETT REMI ARSA ATLI THPF HVIT LRSM VQFI GRES KYCG LCDS IITI YREE GI
Gene ID:	23788
Uniprot:	Q9Y6C9
Cellular Location:	Mitochondrion inner membrane, Multi-pass membrane protein
Calculated MW:	33kDa
Observed MW:	33kDa

Synonyms:

MTCH2, HSPC032, MIMP, SLC25A50

Background:

This gene encodes a member of the SLC25 family of nuclear-encoded transporters that are localized in the inner mitochondrial membrane. Members of this superfamily are involved in many metabolic pathways and cell functions. Genome-wide association studies in human have identified single-nucleotide polymorphisms in several loci associated with obesity. This gene is one such locus, which is highly expressed in white adipose tissue and adipocytes, and thought to play a regulatory role in adipocyte differentiation and biology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA can give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.

UniProt Protein Function:	MTCH2: The substrate transported is not yet known. Induces mitochondrial depolarization. Belongs to the mitochondrial carrier family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: membrane; mitochondrion; nucleus Biological Process: positive regulation of apoptosis
NCBI Summary:	This gene encodes a member of the SLC25 family of nuclear-encoded transporters that are localized in the inner mitochondrial membrane. Members of this superfamily are involved in many metabolic pathways and cell functions. Genome-wide association studies in human have identified single-nucleotide polymorphisms in several loci associated with obesity. This gene is one such locus, which is highly expressed in white adipose tissue and adipocytes, and thought to play a regulatory role in adipocyte differentiation and biology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA can give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]
UniProt Code:	Q9Y6C9
NCBI GenInfo Identifier:	67461088
NCBI Gene ID:	23788
NCBI Accession:	Q9Y6C9.1
UniProt Secondary Accession:	Q9Y6C9,B2R7L8,
UniProt Related Accession:	Q9Y6C9
Molecular Weight:	33kDa
NCBI Full Name:	Mitochondrial carrier homolog 2
NCBI Synonym Full Names:	mitochondrial carrier 2
NCBI Official Symbol:	MTCH2
NCBI Official Synonym Symbols:	MIMP; HSPC032; SLC25A50
NCBI Protein Information:	mitochondrial carrier homolog 2
UniProt Protein Name:	Mitochondrial carrier homolog 2
UniProt Synonym Protein Names:	Met-induced mitochondrial protein
Protein Family:	Mitochondrial carrier
UniProt Gene Name:	MTCH2

	Western blot analysis of extracts of various cell lines, using MTCH2 antibody (CAB12934) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of NIH-3T3 cells using MTCH2 Polyclonal Antibody (CAB12934) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using MTCH2 Polyclonal Antibody (CAB12934) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.