Anti-MTAP Antibody (CAB20907)
- SKU:
- CAB20907
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Universal
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-MTAP Antibody (CAB20907) |
| Antibody SKU: | CAB20907 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human MTAP. |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HT-29, C6, Mouse liver, Mouse kidney, Rat lung, Rat liver, A-549 |
| Immunogen: | Recombinant protein of Human MTAP. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 26kDa/30kDa/31kDa/32kDa/33kDa/36kDa/38kDa |
| Observed MW: | 29KDa |
| Synonyms: | MTAP, BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus |
| Background: | This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. |
 | Immunofluorescence analysis of NIH/3T3 cells using MTAP Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of PC-12 cells using MTAP Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HT-29 cells using MTAP Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using MTAP Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunohistochemistry of paraffin-embedded human placenta using MTAP Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using MTAP Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using MTAP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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