Anti-MTAP Antibody (CAB20907)

Name: Anti-MTAP Antibody (CAB20907)
SKU: CAB20907
Availability: OutOfStock

(No reviews yet) Write a Review

Description

View AllClose

View AllClose

Metabolism Antibodies 1

KO Validated Antibodies 2

OverviewProduct Name:MTAP Polyclonal AntibodyProduct Code:CAB21496Reactivity:Human, Mouse, RatApplications:Western blotting, Immu

OverviewProduct Name:MTAP Monoclonal AntibodyProduct Code:CAB22045Reactivity:Human, Mouse, RatApplications:Western blotting<

Cell Biology Antibodies 14

Cell Biology Antibodies 1

system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक

Antibody Name:	Anti-MTAP Antibody (CAB20907)
Antibody SKU:	CAB20907
Antibody Size:	50µL, 100µL
Application:	Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of Human MTAP.

Application:	Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HT-29, C6, Mouse liver, Mouse kidney, Rat lung, Rat liver, A-549

Immunogen:	Recombinant protein of Human MTAP.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	26kDa/30kDa/31kDa/32kDa/33kDa/36kDa/38kDa
Observed MW:	29KDa

Synonyms:	MTAP, BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus
Background:	This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.

	Immunofluorescence analysis of NIH/3T3 cells using MTAP Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC-12 cells using MTAP Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HT-29 cells using MTAP Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using MTAP Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunohistochemistry of paraffin-embedded human placenta using MTAP Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using MTAP Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Western blot analysis of extracts of various cell lines, using MTAP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.