KO Validated Antibodies 2

Anti-MTAP Antibody (CAB19981)[KO Validated]

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SKU:
CAB19981

Description

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Antibody Name:Anti-MTAP Antibody [KO Validated]
Antibody SKU:CAB19981
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human MTAP.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human MTAP.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4507
Uniprot:Q13126
Cellular Location:Cytoplasm, Nucleus
Calculated MW:26kDa/30kDa/31kDa/32kDa/33kDa/36kDa/38kDa
Observed MW:31KDa
Anti-MTAP Antibody [KO Validated] (CAB19981)Western blot analysis of extracts from normal (control) and MTAP knockout (KO) HeLa cells, using MTAP antibody (CAB19981) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Synonyms:MTAP, BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus
Background:This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.
UniProt Protein Function:MTAP: Catalyzes the reversible phosphorylation of S-methyl-5'- thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S- adenosylmethionine. Has broad substrate specificity with 6- aminopurine nucleosides as preferred substrates. Homotrimer. Ubiquitously expressed. Inhibited by 5'-methylthiotubercin and 5'- chloroformycin. Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - cysteine and methionine; EC 2.4.2.28; Phosphorylase; Transferase

Chromosomal Location of Human Ortholog: 9p21.3

Cellular Component: cytoplasm; cytosol

Molecular Function:phosphorylase activity; protein binding; S-methyl-5-thioadenosine phosphorylase activity

Biological Process: methionine salvage; nicotinamide riboside catabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; purine salvage

NCBI Summary:This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]
UniProt Code:Q13126
NCBI GenInfo Identifier:143811423
NCBI Gene ID:4507
NCBI Accession:Q13126.2
UniProt Secondary Accession:Q13126,Q5T3P3, Q9H010, I2G7M5, I2G7M6, I2G7M7, I2G7M8 I2G7M9, I2G7N0,
UniProt Related Accession:Q13126
Molecular Weight:26,278 Da
NCBI Full Name:S-methyl-5'-thioadenosine phosphorylase
NCBI Synonym Full Names:methylthioadenosine phosphorylase
NCBI Official Symbol:MTAP  
NCBI Official Synonym Symbols:BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249  
NCBI Protein Information:S-methyl-5'-thioadenosine phosphorylase
UniProt Protein Name:S-methyl-5'-thioadenosine phosphorylase
UniProt Synonym Protein Names:5'-methylthioadenosine phosphorylase
UniProt Gene Name:MTAP  
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Additional Information

Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
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