Cell Biology Antibodies 6

Anti-MTA1 Antibody (CAB16085)

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SKU:
CAB16085
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MTA1 Antibody
Antibody SKU:CAB16085
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600-700 of human MTA1 (NP_004680.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, 293T, LO2, MCF-7, Mouse brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600-700 of human MTA1 (NP_004680.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LMPS RGLA NHGQ ARHM GPSR NLLL NGKS YPTK VRLI RGGS LPPV KRRR MNWI DAPD DVFY MATE ETRK IRKL LSSS ETKR AARR PYKP IALR QSQA LPPR P
Gene ID:9112
Uniprot:Q13330
Cellular Location:Cytoplasm, Nucleus, Nucleus envelope, cytoskeleton
Calculated MW:49kDa/79kDa/80kDa
Observed MW:81kDa, 90kDa
Synonyms:MTA1
Background:This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:MTA1: metastasis-associated protein. A component of the NuRD ATP-dependent chromatin remodeling and histone deacetylase complex. Interacts with HDAC1. Expressed at high levels in metastatic cells. Two alternatively spliced isoforms have been described. The long isoform is a co-repressor of estrogen receptor (ER). The short isoform binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of ER.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q32.3

Cellular Component: cytoplasm; intracellular membrane-bound organelle; microtubule; nuclear envelope; nucleoplasm; nucleus; NuRD complex

Molecular Function:chromatin binding; protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity; zinc ion binding

Biological Process: cellular protein metabolic process; circadian regulation of gene expression; double-strand break repair; entrainment of circadian clock by photoperiod; establishment and/or maintenance of chromatin architecture; locomotor rhythm; post-translational protein modification; proteasomal ubiquitin-dependent protein catabolic process; protein sumoylation; regulation of gene expression, epigenetic; regulation of inflammatory response; regulation of transcription, DNA-dependent; response to ionizing radiation; response to lipopolysaccharide; signal transduction; transcription, DNA-dependent

NCBI Summary:This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
UniProt Code:Q13330
NCBI GenInfo Identifier:259016275
NCBI Gene ID:9112
NCBI Accession:Q13330.2
UniProt Secondary Accession:Q13330,Q86SW2, Q8NFI8, Q96GI8, A5PLK4,
UniProt Related Accession:Q13330
Molecular Weight:79,192 Da
NCBI Full Name:Metastasis-associated protein MTA1
NCBI Synonym Full Names:metastasis associated 1
NCBI Official Symbol:MTA1  
NCBI Protein Information:metastasis-associated protein MTA1
UniProt Protein Name:Metastasis-associated protein MTA1
Protein Family:Mating type protein
UniProt Gene Name:MTA1  
UniProt Entry Name:MTA1_HUMAN
Anti-MTA1 Antibody (CAB16085)Western blot analysis of extracts of various cell lines, using MTA1 antibody (CAB16085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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