Cell Biology Antibodies 10
Anti-MSTN Antibody (CAB6913)
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- SKU:
- CAB6913
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MSTN Antibody |
| Antibody SKU: | CAB6913 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 267-375 of human MSTN (NP_005250.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460, U-937, BT-474, Mouse skeletal muscle, Mouse heart, Mouse liver, Mouse gastrocnemius muscle, Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 267-375 of human MSTN (NP_005250.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DFGL DCDE HSTE SRCC RYPL TVDF EAFG WDWI IAPK RYKA NYCS GECE FVFL QKYP HTHL VHQA NPRG SAGP CCTP TKMS PINM LYFN GKEQ IIYG KIPA MVVD RCGC S |
| Gene ID: | 2660 |
| Uniprot: | O14793 |
| Cellular Location: | Secreted |
| Calculated MW: | 42kDa |
| Observed MW: | 43kDa |
| Synonyms: | MSTN, GDF8, MSLHP, myostatin |
| Background: | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. |
| UniProt Protein Function: | MSTN: Acts specifically as a negative regulator of skeletal muscle growth. Defects in MSTN are the cause of muscle hypertrophy (MSLHP). MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong. Belongs to the TGF-beta family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 2q32.2 Cellular Component: extracellular space Molecular Function:cytokine activity; identical protein binding; protein binding; receptor binding; transforming growth factor beta receptor binding Biological Process: cell development; muscle development; muscle maintenance; negative regulation of myoblast differentiation; negative regulation of protein kinase B signaling cascade; negative regulation of skeletal muscle growth; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of MAPKKK cascade Disease: Muscle Hypertrophy |
| UniProt Code: | O14793 |
| NCBI GenInfo Identifier: | 4885259 |
| NCBI Gene ID: | 2660 |
| NCBI Accession: | NP_005250.1 |
| UniProt Secondary Accession: | O14793,Q6B0H2, A1C2J7, A1C2K0, |
| UniProt Related Accession: | O14793 |
| Molecular Weight: | 42,750 Da |
| NCBI Full Name: | growth/differentiation factor 8 preproprotein |
| NCBI Synonym Full Names: | myostatin |
| NCBI Official Symbol: | MSTN |
| NCBI Official Synonym Symbols: | GDF8; MSLHP |
| NCBI Protein Information: | growth/differentiation factor 8 |
| UniProt Protein Name: | Growth/differentiation factor 8 |
| UniProt Synonym Protein Names: | Myostatin |
| Protein Family: | Growth/differentiation factor |
| UniProt Gene Name: | MSTN |
| UniProt Entry Name: | GDF8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MSTN antibody (CAB6913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse lung using MSTN antibody (CAB6913) at dilution of 1:100 (40x lens). |
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