Antibody Name:	Anti-MSH6 Antibody
Antibody SKU:	CAB3177
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human MSH6

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:1000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HeLa, A-549, MCF7, Mouse thymus

Immunogen:	Recombinant protein of human MSH6
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	2956
Uniprot:	P52701
Cellular Location:	Chromosome, Nucleus
Calculated MW:	119kDa/120kDa/137kDa/152kDa
Observed MW:	200kDa

Synonyms:

MSH6, GTBP, GTMBP, HNPCC5, HSAP, p160

Background:

This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described.

UniProt Protein Function:	MSH6: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Belongs to the DNA mismatch repair MutS family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 2p16 Cellular Component: nucleoplasm; Golgi apparatus; nuclear chromosome; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm; plasma membrane; MutSalpha complex Molecular Function:DNA-dependent ATPase activity; protein homodimerization activity; single thymine insertion binding; ATPase activity; oxidized purine DNA binding; magnesium ion binding; ADP binding; methylated histone residue binding; mismatched DNA binding; protein binding; four-way junction DNA binding; single guanine insertion binding; guanine/thymine mispair binding; double-stranded DNA binding; MutLalpha complex binding; chromatin binding; ATP binding Biological Process: negative regulation of DNA recombination; mismatch repair; somatic hypermutation of immunoglobulin genes; isotype switching; determination of adult life span; DNA repair; maintenance of DNA repeat elements; meiotic mismatch repair; positive regulation of helicase activity; DNA damage response, signal transduction resulting in induction of apoptosis; meiotic recombination; somatic recombination of immunoglobulin gene segments; response to UV Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 5; Endometrial Cancer; Mismatch Repair Cancer Syndrome
NCBI Summary:	This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]
UniProt Code:	P52701
NCBI GenInfo Identifier:	68067672
NCBI Gene ID:	2956
NCBI Accession:	P52701.2
UniProt Secondary Accession:	P52701,O43706, O43917, Q8TCX4, Q9BTB5, B4DF41, B4E3I4 F5H2F9,
UniProt Related Accession:	P52701
Molecular Weight:	1360
NCBI Full Name:	DNA mismatch repair protein Msh6
NCBI Synonym Full Names:	mutS homolog 6
NCBI Official Symbol:	MSH6
NCBI Official Synonym Symbols:	GTBP; HSAP; p160; GTMBP; HNPCC5
NCBI Protein Information:	DNA mismatch repair protein Msh6; sperm-associated protein; mutS-alpha 160 kDa subunit; G/T mismatch-binding protein
UniProt Protein Name:	DNA mismatch repair protein Msh6
UniProt Synonym Protein Names:	G/T mismatch-binding protein; GTBP; GTMBP; MutS-alpha 160 kDa subunit; p160
Protein Family:	DNA mismatch repair protein
UniProt Gene Name:	MSH6
UniProt Entry Name:	MSH6_HUMAN

	Western blot analysis of extracts of various cell lines, using MSH6 antibody (CAB3177) at 1:300 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of A549 cells using MSH6 antibody (CAB3177). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using MSH6 antibody (CAB3177). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.