Cell Biology Antibodies 12
Anti-MSH4 Antibody (CAB8556)
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- SKU:
- CAB8556
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MSH4 Antibody |
| Antibody SKU: | CAB8556 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 697-936 of human MSH4 (NP_002431.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse skeletal muscle, Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 697-936 of human MSH4 (NP_002431.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QIMA QIGS YVPA EYSS FRIA KQIF TRIS TDDD IETN SSTF MKEM KEIA YILH NAND KSLI LIDE LGRG TNTE EGIG ICYA VCEY LLSL KAFT LFAT HFLE LCHI DALY PNVE NMHF EVQH VKNT SRNK EAIL YTYK LSKG LTEE KNYG LKAA EVSS LPPS IVLD AKEI TTQI TRQI LQNQ RSTP EMER QRAV YHLA TRLV QTAR NSQL DPDS LRIY LSNL KKKY KEDF PRTE QVPE KTEE |
| Gene ID: | 4438 |
| Uniprot: | O15457 |
| Cellular Location: | |
| Calculated MW: | 104kDa |
| Observed MW: | 105kDa |
| Synonyms: | MSH4 |
| Background: | This gene encodes a member of the DNA mismatch repair mutS family. This member is a meiosis-specific protein that is not involved in DNA mismatch correction, but is required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis I. This protein and MSH5 form a heterodimer which binds uniquely to a Holliday Junction and its developmental progenitor, thus provoking ADP-ATP exchange, and stabilizing the interaction between parental chromosomes during meiosis double-stranded break repair. |
| UniProt Protein Function: | MSH4: Involved in meiotic recombination. Required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis. Belongs to the DNA mismatch repair MutS family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 1p31.1 Cellular Component: nuclear chromosome; nucleus Molecular Function:damaged DNA binding; DNA binding; mismatched DNA binding; protein binding Biological Process: meiotic mismatch repair; meiotic recombination; mismatch repair |
| NCBI Summary: | This gene encodes a member of the DNA mismatch repair mutS family. This member is a meiosis-specific protein that is not involved in DNA mismatch correction, but is required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis I. This protein and MSH5 form a heterodimer which binds uniquely to a Holliday Junction and its developmental progenitor, thus provoking ADP-ATP exchange, and stabilizing the interaction between parental chromosomes during meiosis double-stranded break repair. [provided by RefSeq, Aug 2011] |
| UniProt Code: | O15457 |
| NCBI GenInfo Identifier: | 2463653 |
| NCBI Gene ID: | 4438 |
| NCBI Accession: | AAB72039.1 |
| UniProt Secondary Accession: | O15457,Q5T4U6, Q8NEB3, Q9UNP8, |
| UniProt Related Accession: | O15457 |
| Molecular Weight: | 104,756 Da |
| NCBI Full Name: | MSH4 |
| NCBI Synonym Full Names: | mutS homolog 4 |
| NCBI Official Symbol: | MSH4 |
| NCBI Protein Information: | mutS protein homolog 4 |
| UniProt Protein Name: | MutS protein homolog 4 |
| Protein Family: | MutS protein |
| UniProt Gene Name: | MSH4 |
 | Western blot analysis of extracts of various cell lines, using MSH4 antibody (CAB8556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of OVCAR-3 cells using MSH4 antibody (CAB8556) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse testis using MSH4 antibody (CAB8556) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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