Cell Biology Antibodies 4

Anti-MSH4 Antibody (CAB14027)

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SKU:
CAB14027
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-MSH4 Antibody
Antibody SKU:CAB14027
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 697-936 of human MSH4 (NP_002431.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:SKOV3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 697-936 of human MSH4 (NP_002431.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QIMA QIGS YVPA EYSS FRIA KQIF TRIS TDDD IETN SSTF MKEM KEIA YILH NAND KSLI LIDE LGRG TNTE EGIG ICYA VCEY LLSL KAFT LFAT HFLE LCHI DALY PNVE NMHF EVQH VKNT SRNK EAIL YTYK LSKG LTEE KNYG LKAA EVSS LPPS IVLD AKEI TTQI TRQI LQNQ RSTP EMER QRAV YHLA TRLV QTAR NSQL DPDS LRIY LSNL KKKY KEDF PRTE QVPE KTEE
Gene ID:4438
Uniprot:O15457
Cellular Location:
Calculated MW:104kDa
Observed MW:105kDa
Synonyms:MSH4
Background:This gene encodes a member of the DNA mismatch repair mutS family. This member is a meiosis-specific protein that is not involved in DNA mismatch correction, but is required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis I. This protein and MSH5 form a heterodimer which binds uniquely to a Holliday Junction and its developmental progenitor, thus provoking ADP-ATP exchange, and stabilizing the interaction between parental chromosomes during meiosis double-stranded break repair.
UniProt Protein Function:MSH4: Involved in meiotic recombination. Required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis. Belongs to the DNA mismatch repair MutS family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: nuclear chromosome; nucleus

Molecular Function:damaged DNA binding; DNA binding; mismatched DNA binding; protein binding

Biological Process: meiotic mismatch repair; meiotic recombination; mismatch repair

NCBI Summary:This gene encodes a member of the DNA mismatch repair mutS family. This member is a meiosis-specific protein that is not involved in DNA mismatch correction, but is required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis I. This protein and MSH5 form a heterodimer which binds uniquely to a Holliday Junction and its developmental progenitor, thus provoking ADP-ATP exchange, and stabilizing the interaction between parental chromosomes during meiosis double-stranded break repair. [provided by RefSeq, Aug 2011]
UniProt Code:O15457
NCBI GenInfo Identifier:2463653
NCBI Gene ID:4438
NCBI Accession:AAB72039.1
UniProt Secondary Accession:O15457,Q5T4U6, Q8NEB3, Q9UNP8,
UniProt Related Accession:O15457
Molecular Weight:104,756 Da
NCBI Full Name:MSH4
NCBI Synonym Full Names:mutS homolog 4
NCBI Official Symbol:MSH4  
NCBI Protein Information:mutS protein homolog 4
UniProt Protein Name:MutS protein homolog 4
Protein Family:MutS protein
UniProt Gene Name:MSH4  
Anti-MSH4 Antibody (CAB14027)Western blot analysis of extracts of SKOV3 cells, using MSH4 antibody (CAB14027) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-MSH4 Antibody (CAB14027)Immunofluorescence analysis of rat testis using MSH4 antibody (CAB14027) at dilution of 1:100. Blue: DAPI for nuclear staining.
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