Epigenetics & Nuclear Signaling Antibodies 2
Anti-MSH3 Antibody (CAB5681)
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- SKU:
- CAB5681
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-MSH3 Antibody |
| Antibody SKU: | CAB5681 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 617-761 of human MSH3 (NP_004696.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | SW620 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 617-761 of human MSH3 (NP_004696.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FNTS EEHH ADMY RSDL PNPD TLSA ELHC WRIK WKHR GKDI ELPS TIYE ALHL PDIK FFPN VYAL LKVL CILP VMKV ENER YENG RKRL KAYL RNTL TDQR SSNL ALLN INFD IKHD LDLM VDTY IKLY TSKS ELPT DNSE TVEN T |
| Gene ID: | 4437 |
| Uniprot: | P20585 |
| Cellular Location: | |
| Calculated MW: | 127kDa |
| Observed MW: | 127KDa |
| Synonyms: | MSH3, DUP, FAP4, MRP1 |
| Background: | The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer. |
| UniProt Protein Function: | MSH3: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion- deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Defects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC). Belongs to the DNA mismatch repair MutS family. MSH3 subfamily. |
| UniProt Protein Details: | Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 5q11-q12 Cellular Component: nucleoplasm; nuclear chromosome; membrane; nucleus; MutSbeta complex Molecular Function:DNA-dependent ATPase activity; double-strand/single-strand DNA junction binding; protein homodimerization activity; dinucleotide repeat insertion binding; oxidized purine DNA binding; mismatched DNA binding; loop DNA binding; Y-form DNA binding; centromeric DNA binding; protein binding; enzyme binding; single guanine insertion binding; guanine/thymine mispair binding; dinucleotide insertion or deletion binding; single-stranded DNA binding; ATP binding Biological Process: positive regulation of helicase activity; negative regulation of DNA recombination; mismatch repair; meiotic recombination; somatic recombination of immunoglobulin gene segments; DNA repair; maintenance of DNA repeat elements; meiotic mismatch repair Disease: Endometrial Cancer |
| NCBI Summary: | The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer. [provided by RefSeq, Mar 2011] |
| UniProt Code: | P20585 |
| NCBI GenInfo Identifier: | 317373576 |
| NCBI Gene ID: | 4437 |
| NCBI Accession: | P20585.4 |
| UniProt Secondary Accession: | P20585,Q6PJT5, Q86UQ6, Q92867, A1L480, A1L482, A6NMM6 |
| UniProt Related Accession: | P20585 |
| Molecular Weight: | 1137 |
| NCBI Full Name: | DNA mismatch repair protein Msh3 |
| NCBI Synonym Full Names: | mutS homolog 3 |
| NCBI Official Symbol: | MSH3 |
| NCBI Official Synonym Symbols: | DUP; MRP1 |
| NCBI Protein Information: | DNA mismatch repair protein Msh3; hMSH3; mismatch repair protein 1; divergent upstream protein |
| UniProt Protein Name: | DNA mismatch repair protein Msh3 |
| UniProt Synonym Protein Names: | Divergent upstream protein; DUP; Mismatch repair protein 1; MRP1 |
| Protein Family: | DNA mismatch repair protein |
| UniProt Gene Name: | MSH3 |
| UniProt Entry Name: | MSH3_HUMAN |
 | Western blot analysis of extracts of SW620 cells, using MSH3 Rabbit pAb (CAB5681) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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