Cell Biology Antibodies 12
Anti-MS4A12 Antibody (CAB8223)
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- SKU:
- CAB8223
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | MS4A12 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8223 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human MS4A12 (NP_060186.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa, NCI-H460, BT-474 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human MS4A12 (NP_060186.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MMSS KPTS HAEV NETI PNPY PPSS FMAP GFQQ PLGS INLE NQAQ GAQR AQPY GITS PGIF ASSQ PGQG NIQM INPS VGTA VMNF KEEA KA |
| Gene ID: | 54860 |
| Uniprot: | Q9NXJ0 |
| Cellular Location: | Membrane, Multi-pass membrane protein |
| Calculated MW: | 23kDa/28kDa |
| Observed MW: | 28kDa |
| Synonyms: | MS4A12, Ms4a10 |
| Background: | The protein encoded by this gene is a cell surface protein found primarily in the apical membrane of colonocytes. Silencing of this gene in colon cancer cells inhibits the proliferation, cell motility, and chemotactic invasion of cells. This gene is part of a cluster of similar genes found on chromosome 11. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | MS4A12: May be involved in signal transduction as a component of a multimeric receptor complex. Belongs to the MS4A family. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 11q12 Cellular Component: integral to membrane Molecular Function:protein binding |
| NCBI Summary: | The protein encoded by this gene is a cell surface protein found primarily in the apical membrane of colonocytes. Silencing of this gene in colon cancer cells inhibits the proliferation, cell motility, and chemotactic invasion of cells. This gene is part of a cluster of similar genes found on chromosome 11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009] |
| UniProt Code: | Q9NXJ0 |
| NCBI GenInfo Identifier: | 256600190 |
| NCBI Gene ID: | 54860 |
| NCBI Accession: | NP_001157942.1 |
| UniProt Secondary Accession: | Q9NXJ0,Q8N6L4, F5GX98, |
| UniProt Related Accession: | Q9NXJ0 |
| Molecular Weight: | 35kD |
| NCBI Full Name: | membrane-spanning 4-domains subfamily A member 12 isoform 2 |
| NCBI Synonym Full Names: | membrane spanning 4-domains A12 |
| NCBI Official Symbol: | MS4A12 |
| NCBI Official Synonym Symbols: | Ms4a10 |
| NCBI Protein Information: | membrane-spanning 4-domains subfamily A member 12 |
| UniProt Protein Name: | Membrane-spanning 4-domains subfamily A member 12 |
| Protein Family: | Membrane-spanning 4-domains subfamily |
| UniProt Gene Name: | MS4A12 |
| UniProt Entry Name: | M4A12_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MS4A12 antibody (CAB8223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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