Antibody Name:	Anti-MRPS6 Antibody
Antibody SKU:	CAB9884
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human MRPS6 (NP_115865.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	B cells, MCF7, 22Rv1, Jurkat, Mouse kidney, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human MRPS6 (NP_115865.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPRY ELAL ILKA MQRP ETAA TLKR TIEA LMDR GAIV RDLE NLGE RALP YRIS AHSQ QHNR GGYF LVDF YAPT AAVE SMVE HLSR DIDV IRGN IVKH PLTQ ELKE CEGI VPVP LAEK LYST KKRK K
Gene ID:	64968
Uniprot:	P82932
Cellular Location:	Mitochondrion
Calculated MW:	14kDa
Observed MW:	14kDa

Synonyms:

MRPS6, C21orf101, MRP-S6, RPMS6, S6mt

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S6P family. Pseudogenes corresponding to this gene are found on chromosomes 1p and 12q.

UniProt Protein Function:	MRPS6: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S6P family. Pseudogenes corresponding to this gene are found on chromosomes 1p and 12q. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 21q22.11 Cellular Component: mitochondrial inner membrane; mitochondrial small ribosomal subunit Molecular Function:protein binding; structural constituent of ribosome Biological Process: mitochondrial translation
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S6P family. Pseudogenes corresponding to this gene are found on chromosomes 1p and 12q. [provided by RefSeq, Jul 2008]
UniProt Code:	P82932
NCBI GenInfo Identifier:	16554616
NCBI Gene ID:	64968
NCBI Accession:	NP_115865.1
UniProt Secondary Accession:	P82932,Q96Q64, Q9BSK8, Q9BW89, B2R573,
UniProt Related Accession:	P82932
Molecular Weight:	14,227 Da
NCBI Full Name:	28S ribosomal protein S6, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein S6
NCBI Official Symbol:	MRPS6
NCBI Official Synonym Symbols:	S6mt; RPMS6; MRP-S6; C21orf101
NCBI Protein Information:	28S ribosomal protein S6, mitochondrial
UniProt Protein Name:	28S ribosomal protein S6, mitochondrial
Protein Family:	28S ribosomal protein
UniProt Gene Name:	MRPS6
UniProt Entry Name:	RT06_HUMAN

	Western blot analysis of extracts of various cell lines, using MRPS6 antibody (CAB9884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded mouse heart using MRPS6 antibody (CAB9884) at dilution of 1:100 (40x lens).