Name: Anti-MRPS33 Antibody (CAB14893)
Brand: Cell Biology Antibodies 5
SKU: CAB14893
Availability: OutOfStock

Antibody Name:	Anti-MRPS33 Antibody
Antibody SKU:	CAB14893
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human MRPS33 (NP_057155.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	A-549, 293T, Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human MRPS33 (NP_057155.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSSL SEYA FRMS RLSA RLFG EVTR PTNS KSMK VVKL FSEL PLAK KKET YDWY PNHH TYAE LMQT LRFL GLYR DEHQ DFMD EQKR LKKL RGKE KPKK GEGK RAAK RK
Gene ID:	51650
Uniprot:	Q9Y291
Cellular Location:	Mitochondrion
Calculated MW:	12kDa
Observed MW:	13kDa

Synonyms:

MRPS33, CGI-139, MRP-S33, PTD003, S33mt

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that is one of the more highly conserved mitochondrial ribosomal proteins among mammals, Drosophila and C. elegans. Splice variants that differ in the 5' UTR have been found for this gene; all variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q, 4p, 4q, and 20q

UniProt Protein Function:	MRPS33: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.
UniProt Protein Details:	Protein type:Mitochondrial; Ribosomal Chromosomal Location of Human Ortholog: 7q34 Cellular Component: mitochondrial inner membrane; mitochondrion
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that is one of the more highly conserved mitochondrial ribosomal proteins among mammals, Drosophila and C. elegans. Splice variants that differ in the 5' UTR have been found for this gene; all variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q, 4p, 4q, and 20q [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y291
NCBI GenInfo Identifier:	24212388
NCBI Gene ID:	51650
NCBI Accession:	Q9Y291.1
UniProt Related Accession:	Q9Y291
Molecular Weight:	12,629 Da
NCBI Full Name:	28S ribosomal protein S33, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein S33
NCBI Official Symbol:	MRPS33
NCBI Official Synonym Symbols:	S33mt; PTD003; CGI-139; MRP-S33
NCBI Protein Information:	28S ribosomal protein S33, mitochondrial
UniProt Protein Name:	28S ribosomal protein S33, mitochondrial
Protein Family:	28S ribosomal protein
UniProt Gene Name:	MRPS33
UniProt Entry Name:	RT33_HUMAN

Western blot analysis of extracts of various cell lines, using MRPS33 antibody (CAB14893) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

MRPS33 Antibody (PACO04562)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)