Antibody Name:	MRPS22 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8319
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-360 of human MRPS22 (NP_064576.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, HeLa, SW480, A-549, MCF7, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-360 of human MRPS22 (NP_064576.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPL GTTV LLWS LLRS SPGV ERVC FRAR IQPW HGGL LQPL PCSF EMGL PRRR FSSE AAES GSPE TKKP TFMD EEVQ SILT KMTG LNLQ KTFK PAIQ ELKP PTYK LMTQ AQLE EATR QAVE AAKV RLKM PPVL EERV PIND VLAE DKIL EGTE TTKY VFTD ISYS IPHR ERFI VVRE PSGT LRKA SWEE RDRM IQVY FPKE GRKI LTPI IFKE ENLR TMYS QDRH VDVL NLCF AQFE PDST EYIK VHHK TYED IDKR GKYD LLRS TRYF GGMV WYFV NNKK IDGL LIDQ IQRD LIDD ATNL VQLY HVLH PDGQ SAQG AKDQ AAEG INLI KVFA KTEA QKGA YIEL TLQT YQEA LSRH SAAS
Gene ID:	56945
Uniprot:	P82650
Cellular Location:	Mitochondrion
Calculated MW:	18kDa/41kDa
Observed MW:	41kDa

Synonyms:

MRPS22, C3orf5, COXPD5, GIBT, GK002, MRP-S22, RPMS22

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. This gene lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene corresponding to this gene is found on chromosome Xq.

UniProt Protein Function:	MRPS22: Defects in MRPS22 are the cause of combined oxidative phosphorylation deficiency type 5 (COXPD5). COXPD5 is an antenatal mitochondrial disease. Patients show edema, cardiomyopathy, tubulopathy, and hypotonia.
UniProt Protein Details:	Protein type:Mitochondrial; Ribosomal; Translation Chromosomal Location of Human Ortholog: 3q23 Cellular Component: mitochondrial inner membrane; mitochondrial ribosome; mitochondrial small ribosomal subunit; mitochondrion Molecular Function:structural constituent of ribosome Biological Process: mitochondrial translation; organelle organization and biogenesis Disease: Combined Oxidative Phosphorylation Deficiency 5
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. This gene lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene corresponding to this gene is found on chromosome Xq. [provided by RefSeq, Jul 2008]
UniProt Code:	P82650
NCBI GenInfo Identifier:	13633893
NCBI Gene ID:	56945
NCBI Accession:	P82650.1
UniProt Secondary Accession:	P82650,Q9H3I1,
UniProt Related Accession:	P82650
Molecular Weight:	18,972 Da
NCBI Full Name:	28S ribosomal protein S22, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein S22
NCBI Official Symbol:	MRPS22
NCBI Official Synonym Symbols:	GIBT; GK002; C3orf5; COXPD5; RPMS22; MRP-S22
NCBI Protein Information:	28S ribosomal protein S22, mitochondrial
UniProt Protein Name:	28S ribosomal protein S22, mitochondrial
Protein Family:	28S ribosomal protein
UniProt Gene Name:	MRPS22
UniProt Entry Name:	RT22_HUMAN

	Western blot analysis of extracts of various cell lines, using MRPS22 antibody (CAB8319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using MRPS22 antibody (CAB8319) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using MRPS22 antibody (CAB8319) at dilution of 1:100 (40x lens).