Name: Anti-MRPS2 Antibody (CAB14279)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB14279
Availability: OutOfStock

Antibody Name:	Anti-MRPS2 Antibody
Antibody SKU:	CAB14279
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-296 of human MRPS2 (NP_057118.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Mouse
Positive Samples:	Mouse brain, Mouse heart, Mouse kidney, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-296 of human MRPS2 (NP_057118.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATS SAAL PRIL GAGA RAPS RWLG FLGK ATPR PARP SRRT LGSA TALM IRES EDST DFND KILN EPLK HSDF FNVK ELFS VRSL FDAR VHLG HKAG CRHR FMEP YIFG SRLD HDII DLEQ TATH LQLA LNFT AHMA YRKG IILF ISRN RQFS YLIE NMAR DCGE YAHT RYFR GGML TNAR LLFG PTVR LPDL IIFL HTLN NIFE PHVA VRDA AKMN IPTV GIVD TNCN PCLI TYPV PGND DSPL AVHL YCRL FQTA ITRA KEKR QQVE ALYR LQGQ KEPG DQGP AHPP GADM SHSL
Gene ID:	51116
Uniprot:	Q9Y399
Cellular Location:	Mitochondrion
Calculated MW:	33kDa
Observed MW:	33kDa

Synonyms:

MRPS2, CGI-91, MRP-S2, S2mt

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S2 family. Alternatively spliced transcript variants have been observed for this gene.

UniProt Protein Function:	MRPS2: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S2 family. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
UniProt Protein Details:	Protein type:Mitochondrial; Ribosomal; Translation Chromosomal Location of Human Ortholog: 9q34 Cellular Component: mitochondrial small ribosomal subunit; mitochondrion Molecular Function:structural constituent of ribosome Biological Process: mitochondrial translation; positive regulation of defense response to virus by host; translation
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S2 family. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
UniProt Code:	Q9Y399
NCBI GenInfo Identifier:	24212389
NCBI Gene ID:	51116
NCBI Accession:	Q9Y399.1
UniProt Secondary Accession:	Q9Y399,Q5T899, Q9BSQ4,
UniProt Related Accession:	Q9Y399
Molecular Weight:	33,249 Da
NCBI Full Name:	28S ribosomal protein S2, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein S2
NCBI Official Symbol:	MRPS2
NCBI Official Synonym Symbols:	S2mt; CGI-91; MRP-S2
NCBI Protein Information:	28S ribosomal protein S2, mitochondrial
UniProt Protein Name:	28S ribosomal protein S2, mitochondrial
Protein Family:	28S ribosomal protein
UniProt Gene Name:	MRPS2
UniProt Entry Name:	RT02_HUMAN

Western blot analysis of extracts of various cell lines, using MRPS2 antibody (CAB14279) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

MRPS2 Antibody (PACO04519)

MRPS2 Antibody (PACO10604)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)