Antibody Name:	Anti-MRPS16 Antibody
Antibody SKU:	CAB9874
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human MRPS16 (NP_057149.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, SW620, HT-1080, MCF7, Mouse heart, Mouse kidney, Mouse liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human MRPS16 (NP_057149.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVHL TTLL CKAY RGGH LTIR LALG GCTN RPFY RIVA AHNK CPRD GRFV EQLG SYDP LPNS HGEK LVAL NLDR IRHW IGCG AHLS KPME KLLG LAGF FPLH PMMI TNAE RLRR KRAR EVLL ASQK TDAE ATDT EATE T
Gene ID:	51021
Uniprot:	Q9Y3D3
Cellular Location:	Mitochondrion
Calculated MW:	13kDa/15kDa
Observed MW:	15kDa

Synonyms:

MRPS16, CGI-132, COXPD2, MRP-S16, RPMS16

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S16P family. The encoded protein is one of the most highly conserved ribosomal proteins between mammalian and yeast mitochondria. Three pseudogenes (located at 8q21.3, 20q13.32, 22q12-q13.1) for this gene have been described.

UniProt Protein Function:	MRPS16: Defects in MRPS16 are the cause of combined oxidative phosphorylation deficiency type 2 (COXPD2). Defects in the mitochondrial oxidative phosphorylation system result in devastating, mainly multisystem, diseases. COXPD2 symptoms include fatal neonatal metabolic acidosis with agenesis of the corpus callosum. Belongs to the ribosomal protein S16P family.
UniProt Protein Details:	Protein type:Mitochondrial; Ribosomal; Translation Chromosomal Location of Human Ortholog: 10q22.1 Cellular Component: mitochondrion; mitochondrial small ribosomal subunit; cytoplasm; mitochondrial inner membrane Molecular Function:protein binding; structural constituent of ribosome Biological Process: translation; mitochondrial translation; organelle organization and biogenesis Disease: Combined Oxidative Phosphorylation Deficiency 2
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S16P family. The encoded protein is one of the most highly conserved ribosomal proteins between mammalian and yeast mitochondria. Three pseudogenes (located at 8q21.3, 20q13.32, 22q12-q13.1) for this gene have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y3D3
NCBI GenInfo Identifier:	7705626
NCBI Gene ID:	51021
NCBI Accession:	NP_057149.1
UniProt Secondary Accession:	Q9Y3D3,Q96Q60, B4E032,
UniProt Related Accession:	Q9Y3D3
Molecular Weight:	13,829 Da
NCBI Full Name:	28S ribosomal protein S16, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein S16
NCBI Official Symbol:	MRPS16
NCBI Official Synonym Symbols:	COXPD2; RPMS16; CGI-132; MRP-S16
NCBI Protein Information:	28S ribosomal protein S16, mitochondrial
UniProt Protein Name:	28S ribosomal protein S16, mitochondrial
Protein Family:	28S ribosomal protein
UniProt Gene Name:	MRPS16
UniProt Entry Name:	RT16_HUMAN

	Western blot analysis of extracts of various cell lines, using MRPS16 antibody (CAB9874) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Confocal immunofluorescence analysis of U2OS cells using Anti-MRPS16 Polyclonal Antibody (CAB9874) at dilution of 1:100. Blue: DAPI for nuclear staining.