Name: Anti-MRPS12 Antibody (CAB10573)
Brand: Cell Biology Antibodies 2
SKU: CAB10573
Availability: OutOfStock

Antibody Name:	Anti-MRPS12 Antibody
Antibody SKU:	CAB10573
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-138 of human MRPS12 (NP_203527.1).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human
Positive Samples:	LO2, U-251MG, HT-29, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-138 of human MRPS12 (NP_203527.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATL NQMH RLGP PKRP PRKL GPTE GRPQ LKGV VLCT FTRK PKKP NSAN RKCC RVRL STGR EAVC FIPG EGHT LQEH QIVL VEGG RTQD LPGV KLTV VRGK YDCG HVQK K
Gene ID:	6183
Uniprot:	O15235
Cellular Location:	Mitochondrion
Calculated MW:	15kDa
Observed MW:	14kDa

Synonyms:

MRPS12, MPR-S12, MT-RPS12, RPMS12, RPS12, RPSM12

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein.

UniProt Protein Function:	MRPS12: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: mitochondrial inner membrane; mitochondrial ribosome; mitochondrial small ribosomal subunit Molecular Function:protein binding; RNA binding; structural constituent of ribosome Biological Process: mitochondrial translation; translation
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein. [provided by RefSeq, Jul 2008]
UniProt Code:	O15235
NCBI GenInfo Identifier:	3024582
NCBI Gene ID:	6183
NCBI Accession:	O15235.1
UniProt Secondary Accession:	O15235,Q53X98,
UniProt Related Accession:	O15235
Molecular Weight:	15kDa
NCBI Full Name:	28S ribosomal protein S12, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein S12
NCBI Official Symbol:	MRPS12
NCBI Official Synonym Symbols:	RPS12; RPMS12; RPSM12; MPR-S12; MT-RPS12
NCBI Protein Information:	28S ribosomal protein S12, mitochondrial
UniProt Protein Name:	28S ribosomal protein S12, mitochondrial
UniProt Synonym Protein Names:	MT-RPS12; Mitochondrial small ribosomal subunit protein uS12m
Protein Family:	28S ribosomal protein
UniProt Gene Name:	MRPS12

Western blot analysis of extracts of various cell lines, using MRPS12 antibody (CAB10573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

MRPS12 Antibody (PACO06655)

MRPS12 Antibody (PACO10598)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)