Epigenetics & Nuclear Signaling Antibodies 2
Anti-MRPL54 Antibody (CAB14957)
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- SKU:
- CAB14957
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-MRPL54 Antibody |
| Antibody SKU: | CAB14957 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human MRPL54 (NP_758455.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | A-431, HT-29, LO2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human MRPL54 (NP_758455.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MATK RLFG ATRT WAGW GAWE LLNP ATSG RLLA RDYA KKPV MKGA KSGK GAVT SEAL KDPD VCTD PVQL TTYA MGVN IYKE GQDV PLKP DAEY PEWL FEMN LGPP KTLE ELDP ESRE YWRR LRKQ NIWR HNRL SKNK RL |
| Gene ID: | 116541 |
| Uniprot: | Q6P161 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 15kDa |
| Observed MW: | 15kDa |
| Synonyms: | MRPL54, L54mt, MRP-L54 |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. |
| UniProt Protein Function: | MRPL54: |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: mitochondrial inner membrane; ribosome Biological Process: mitochondrial translation; organelle organization and biogenesis |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q6P161 |
| NCBI GenInfo Identifier: | 74737109 |
| NCBI Gene ID: | 116541 |
| NCBI Accession: | Q6P161.1 |
| UniProt Related Accession: | Q6P161 |
| Molecular Weight: | 15,819 Da |
| NCBI Full Name: | 39S ribosomal protein L54, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein L54 |
| NCBI Official Symbol: | MRPL54 |
| NCBI Official Synonym Symbols: | L54mt; MRP-L54 |
| NCBI Protein Information: | 39S ribosomal protein L54, mitochondrial |
| UniProt Protein Name: | 39S ribosomal protein L54, mitochondrial |
| Protein Family: | 39S ribosomal protein |
| UniProt Gene Name: | MRPL54 |
| UniProt Entry Name: | RM54_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MRPL54 antibody (CAB14957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded Human colon using MRPL54 Rabbit pAb (CAB14957) at dilution of 1:100 (40x lens). |
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