Antibody Name:	Anti-MRPL46 Antibody
Antibody SKU:	CAB15428
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-279 of human MRPL46 (NP_071446.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-279 of human MRPL46 (NP_071446.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAP VRRT LLGV AGGW RRFE RLWA GSLS SRSL ALAA APSS NGSP WRLL GALC LQRP PVVS KPLT PLQE EMAS LLQQ IEIE RSLY SDHE LRAL DENQ RLAK KKAD LHDE EDEQ DILL AQDL EDMW EQKF LQFK LGAR ITEA DEKN DRTS LNRK LDRN LVLL VREK FGDQ DVWI LPQA EWQP GETL RGTA ERTL ATLS ENNM EAKF LGNA PCGH YTFK FPQA MRTE SNLG AKVF FFKA LLLT GDFS QAGN KGHH VWVT KDEL GDYL KPKY LAQV RRFV SDL
Gene ID:	26589
Uniprot:	Q9H2W6
Cellular Location:	Mitochondrion
Calculated MW:	31kDa
Observed MW:	32kDa

Synonyms:

MRPL46, C15orf4, LIECG2, P2ECSL

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.

UniProt Protein Function:	MRPL46: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Ribosomal; Translation; Mitochondrial Chromosomal Location of Human Ortholog: 15q25.3 Cellular Component: cell junction; mitochondrial inner membrane; mitochondrial large ribosomal subunit; mitochondrion; nucleoplasm Molecular Function:structural constituent of ribosome
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9H2W6
NCBI GenInfo Identifier:	52783325
NCBI Gene ID:	26589
NCBI Accession:	Q9H2W6.1
UniProt Secondary Accession:	Q9H2W6,Q9HBU8, B2RD75,
UniProt Related Accession:	Q9H2W6
Molecular Weight:	31,705 Da
NCBI Full Name:	39S ribosomal protein L46, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein L46
NCBI Official Symbol:	MRPL46
NCBI Official Synonym Symbols:	LIECG2; P2ECSL; C15orf4
NCBI Protein Information:	39S ribosomal protein L46, mitochondrial
UniProt Protein Name:	39S ribosomal protein L46, mitochondrial
UniProt Synonym Protein Names:	P2ECSL
Protein Family:	39S ribosomal protein
UniProt Gene Name:	MRPL46
UniProt Entry Name:	RM46_HUMAN

	Western blot analysis of extracts of mouse brain, using MRPL46 antibody (CAB15428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using MRPL46 antibody (CAB15428) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lymph node using MRPL46 antibody (CAB15428) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using MRPL46 antibody (CAB15428) at dilution of 1:100 (40x lens).