Name: Anti-MRPL42 Antibody (CAB12811)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB12811
Availability: OutOfStock

Antibody Name:	Anti-MRPL42 Antibody
Antibody SKU:	CAB12811
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human MRPL42 (NP_054769.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	U-87MG, HeLa, Raji, HepG2, U-251MG, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human MRPL42 (NP_054769.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAVA AVKW VMSK RTIL KHLF PVQN GALY CVCH KSTY SPLP DDYN CNVE LALT SDGR TIVC YHPS VDIP YEHT KPIP RPDP VHNN EETH DQVL KTRL EEKV EHLE EGPM IEQL SKMF FTTK HRWY PHGR YHRC RKNL NPPK DR
Gene ID:	28977
Uniprot:	Q9Y6G3
Cellular Location:	Mitochondrion
Calculated MW:	16kDa
Observed MW:	16kDa

Synonyms:

MRPL42, HSPC204, L31MT, L42MT, MRP-L31, MRP-L42, MRP-S32, MRPL31, MRPS32, PTD007, RPML31, S32MT

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q.

UniProt Protein Function:	MRPL42: Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins. Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.
UniProt Protein Details:	Protein type:Ribosomal; Mitochondrial Chromosomal Location of Human Ortholog: 12q22 Cellular Component: mitochondrial inner membrane; mitochondrial small ribosomal subunit; mitochondrion; plasma membrane Molecular Function:structural constituent of ribosome Biological Process: mitochondrial translation; organelle organization and biogenesis; translation
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provided by RefSeq, May 2011]
UniProt Code:	Q9Y6G3
NCBI GenInfo Identifier:	7662637
NCBI Gene ID:	28977
NCBI Accession:	NP_054769.1
UniProt Secondary Accession:	Q9Y6G3,Q6FID1, Q96Q48, Q9P0S1,
UniProt Related Accession:	Q9Y6G3
Molecular Weight:	16,661 Da
NCBI Full Name:	39S ribosomal protein L42, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein L42
NCBI Official Symbol:	MRPL42
NCBI Official Synonym Symbols:	L31MT; L42MT; S32MT; MRPL31; MRPS32; PTD007; RPML31; HSPC204; MRP-L31; MRP-L42; MRP-S32
NCBI Protein Information:	39S ribosomal protein L42, mitochondrial
UniProt Protein Name:	39S ribosomal protein L42, mitochondrial
UniProt Synonym Protein Names:	28S ribosomal protein S32, mitochondrial; MRP-S32; S32mt; 39S ribosomal protein L31, mitochondrial; L31mt; MRP-L31
Protein Family:	39S ribosomal protein
UniProt Gene Name:	MRPL42
UniProt Entry Name:	RM42_HUMAN

Western blot analysis of extracts of various cell lines, using MRPL42 antibody (CAB12811) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

MRPL42 Antibody (PACO03972)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)