Cell Biology Antibodies 1

Anti-MRPL32 Antibody (CAB10016)

(No reviews yet) Write a Review
SKU:
CAB10016
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-MRPL32 Antibody
Antibody SKU:CAB10016
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-188 of human MRPL32 (NP_114109.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, Raji, MCF7, HepG2, A-549, Mouse heart, Mouse kidney, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-188 of human MRPL32 (NP_114109.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VLRN YWER LLRK LPQS RPGF PSPP WGPA LAVQ GPAM FTEP ANDT SGSK ENSS LLDS IFWM AAPK NRRT IEVN RCRR RNPQ KLIK VKNN IDVC PECG HLKQ KHVL CAYC YEKV CKET AEIR RQIG KQEG GPFK APTI ETVV LYTG ETPS EQDQ GKRI IERD RKRP SWFT QN
Gene ID:64983
Uniprot:Q9BYC8
Cellular Location:Mitochondrion
Calculated MW:21kDa
Observed MW:14kDa
Synonyms:MRPL32, HSPC283, L32mt, MRP-L32, bMRP-59b
Background:Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.
UniProt Protein Function:MRPL32: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: mitochondrial inner membrane; mitochondrial ribosome; large ribosomal subunit

Molecular Function:structural constituent of ribosome

Biological Process: mitochondrial translation; translation; organelle organization and biogenesis

NCBI Summary:Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BYC8
NCBI GenInfo Identifier:13994261
NCBI Gene ID:64983
NCBI Accession:NP_114109.1
UniProt Secondary Accession:Q9BYC8,Q96Q68, Q9P098,
UniProt Related Accession:Q9BYC8
Molecular Weight:21,405 Da
NCBI Full Name:39S ribosomal protein L32, mitochondrial
NCBI Synonym Full Names:mitochondrial ribosomal protein L32
NCBI Official Symbol:MRPL32  
NCBI Official Synonym Symbols:L32mt; HSPC283; MRP-L32; bMRP-59b  
NCBI Protein Information:39S ribosomal protein L32, mitochondrial
UniProt Protein Name:39S ribosomal protein L32, mitochondrial
Protein Family:39S ribosomal protein
UniProt Gene Name:MRPL32  
UniProt Entry Name:RM32_HUMAN
Anti-MRPL32 Antibody (CAB10016)Western blot analysis of extracts of various cell lines, using MRPL32 antibody (CAB10016) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
View AllClose

0 Reviews

View AllClose

Related Products