Antibody Name:	Anti-MRPL23 Antibody
Antibody SKU:	CAB16424
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human MRPL23 (NP_066957.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, U-251MG, A-549, Mouse liver, Mouse lung, Mouse brain, Rat heart, Rat liver, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human MRPL23 (NP_066957.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MARN VVYP LYRL GGPQ LRVF RTNF FIQL VRPG VAQP EDTV QFRI PMEM TRVD LRNY LEGI YNVP VAAV RTRV QHGS NKRR DHRN VRIK KPDY KVAY VQLA HGQT FTFP DLFP EKDE SPEG SAAD DLYS MLEE ERQQ RQSS DPRR GGVP SWFG L
Gene ID:	6150
Uniprot:	Q16540
Cellular Location:	Mitochondrion
Calculated MW:	17kDa
Observed MW:	15kDa

Synonyms:

MRPL23, L23MRP, RPL23, RPL23L

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. The gene is biallelically expressed, despite its location within a region of imprinted genes on chromosome 11.

UniProt Protein Function:	MRPL23: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. The gene is biallelically expressed, despite its location within a region of imprinted genes on chromosome 11. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Mitochondrial; Ribosomal; RNA-binding Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: mitochondrion; mitochondrial inner membrane; nucleolus; mitochondrial large ribosomal subunit Molecular Function:structural constituent of ribosome; RNA binding; nucleotide binding Biological Process: mitochondrial translation; translation; organelle organization and biogenesis
UniProt Code:	Q16540
NCBI GenInfo Identifier:
NCBI Gene ID:	1433
NCBI Accession:	NP_001306.2
UniProt Secondary Accession:	Q16540,Q96Q71, A8MT29,
Molecular Weight:	17,781 Da
NCBI Full Name:
NCBI Synonym Full Names:	choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity)
NCBI Official Symbol:	CSE
NCBI Official Synonym Symbols:	DYT9
UniProt Protein Name:	39S ribosomal protein L23, mitochondrial
UniProt Synonym Protein Names:	L23 mitochondrial-related protein; Ribosomal protein L23-like
Protein Family:	Caffeoylshikimate esterase
UniProt Gene Name:	MRPL23
UniProt Entry Name:	RM23_HUMAN

	Western blot analysis of extracts of various cell lines, using MRPL23 antibody (CAB16424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human thyroid cancer using MRPL23 antibody (CAB16424) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using MRPL23 antibody (CAB16424) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using MRPL23 antibody (CAB16424) at dilution of 1:100. Blue: DAPI for nuclear staining.