Name: Anti-MRPL22 Antibody (CAB14586)
Brand: Cell Biology Antibodies 5
SKU: CAB14586
Availability: OutOfStock

Antibody Name:	Anti-MRPL22 Antibody
Antibody SKU:	CAB14586
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 97-206 of human MRPL22 (NP_054899.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Mouse heart, Mouse kidney, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 97-206 of human MRPL22 (NP_054899.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LAQL EFND KKGA KIIK EVLL EAQD MAVR DHNV EFRS NLYI AEST SGRG QCLK RIRY HGRG RFGI MEKV YCHY FVKL VEGP PPPP EPPK TAVA HAKE YIQQ LRSR TIVH TL
Gene ID:	29093
Uniprot:	Q9NWU5
Cellular Location:	Mitochondrion
Calculated MW:	13kDa/14kDa/23kDa
Observed MW:	24kDa

Synonyms:

MRPL22, HSPC158, L22mt, MRP-L22, MRP-L25, RPML25

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	MRPL22: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Translation Chromosomal Location of Human Ortholog: 5q33.2 Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit; mitochondrion Molecular Function:structural constituent of ribosome Biological Process: mitochondrial translation; organelle organization and biogenesis
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NWU5
NCBI GenInfo Identifier:	74753031
NCBI Gene ID:	29093
NCBI Accession:	Q9NWU5.1
UniProt Secondary Accession:	Q9NWU5,Q5H9Q1, Q96Q51, Q9P006, A6NGJ8,
UniProt Related Accession:	Q9NWU5
Molecular Weight:	14,509 Da
NCBI Full Name:	39S ribosomal protein L22, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein L22
NCBI Official Symbol:	MRPL22
NCBI Official Synonym Symbols:	L22mt; RPML25; HSPC158; MRP-L22; MRP-L25
NCBI Protein Information:	39S ribosomal protein L22, mitochondrial
UniProt Protein Name:	39S ribosomal protein L22, mitochondrial
UniProt Synonym Protein Names:	39S ribosomal protein L25, mitochondrial; L25mt; MRP-L25
Protein Family:	39S ribosomal protein
UniProt Gene Name:	MRPL22
UniProt Entry Name:	RM22_HUMAN

Western blot analysis of extracts of various cell lines, using MRPL22 antibody (CAB14586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

MRPL22 Antibody (PACO03989)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)